Rapid and sensitive detection of urinary KIM-1 using fully printed photonic crystal microarrays  

作　　者：Yang Liu Xuwei He Zewei Lian Qian Guo Jimei Chi Xiaoxue Lin Liyue Zhang Zheng Liu Yingyuan Liu Meng Su Keyu Wang Qiangguo Ao Qingli Cheng 

机构地区：[1]Department of Nephrology,The Second Medical Center of Chinese PLA General Hospital,National Clinical Research Centre for Geriatric Diseases,Beijing 100853,China [2]Chinese PLA Medical School,Beijing 100853,China [3]Key Laboratory of Green Printing,CAS Research/Education Center for Excellence in Molecular Sciences,Beijing Engineering Research Center of Nanomaterials for Green Printing Technology,Beijing National Laboratory for Molecular Sciences(BNLMS),Institute of Chemistry,Chinese Academy of Sciences(ICCAS),Beijing 100190,China [4]Department of Rheumatology and Immunology,Peking University International Hospital,Beijing 102206,China [5]Department of Clinical Laboratory,The Second Medical Center of Chinese PLA General Hospital,National Clinical Research Centre for Geriatric Diseases,Beijing 100853,China

出　　处：《Nano Research》2024年第5期4329-4337,共9页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82170684,52222313,22075296,91963212,82000004);the Health Care Program Foundation of PLA(No.21BJZ17);the Youth Independent Innovation Science Fund of the General Hospital of the People’s Liberation Army(No.22QNFC007);the Youth Innovation Promotion Association CAS(No.2020032);the Intramural Research Fund of Peking University International Hospital(No.YN2021QN05).

摘　　要：Urinary kidney injury molecule 1(uKIM-1)serves as a reliable marker for the early diagnosis of acute kidney injury(AKI).The rapid and facile detection of changes in uKIM-1 is essential for early AKI diagnosis,ultimately improving the prognosis of patients.In this study,we developed a fully printed photonic crystal-integrated microarray with photonic crystal-enhanced fluorescence properties,which can detect uKIM-1 levels at the point-of-care.We confirmed its efficacy in the early diagnosis of AKI using clinical urine specimens.Direct quantitative detection of uKIM-1 was achieved within 10 min.The lowest limit of detection is 8.75 pg·mL^(-1) with an accuracy of 94.2%.The diagnostic efficacy was validated using 86 clinical urine samples,highlighting the high sensitivity and stability of the photonic crystal microarray.Consequently,a facile and reliable immunoassay was designed and prepared for the rapid quantitative detection of uKIM-1,which is crucial for the early identification and convenient detection of AKI in hospital or community settings.Rapid,convenient,cost-effective,and long-term monitoring of changes in uKIM-1 levels can assist clinicians in making timely adjustments to treatment regimens,preventing the transition from AKI to chronic kidney disease(CKD),improving the quality of life of patients with AKI,and reducing healthcare costs.It highlights the advantages of utilizing urine samples as a noninvasive and easily accessible medium for early detection and monitoring of kidney-related conditions.

关 键 词：photonic crystal point-of-care testing URINE kidney injury molecule-1(KIM-1) acute kidney injury 

分 类 号：O734[理学—晶体学]