UPLC-Q-Orbitrap-MS快速分析水红花子的化学成分  

Rapid identification of the chemical constituents in the fructus of Polygounum orientale by UPLC-Q-Orbitrap-MS

作　　者：边星 夏林波[1] 邓仕任[1] 孙璐 杨浩 BIAN Xing;XIA Linbo;DENG Shiren;SUN Lu;YANG Hao(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《药学研究》2024年第3期243-249,287,共8页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(No.81202857);辽宁省自然科学基金(No.20170540617)。

摘　　要：目的建立水红花子化学成分的快速鉴定方法。方法利用超高效液相串联四极杆静电场轨道阱质谱(UPLC-Q-Orbitrap-MS)技术,正、负离子模式下分别采集一级、二级质谱信息。通过推导质谱裂解规律,与质谱数据库(MassBank、mzCloud)、文献数据、对照品比对等方式,对化合物进行指认。结果从水红花子中共鉴定出54种化学成分,主要包括黄酮类、酚酸类、生物碱类等,其中有15种成分为首次从水红花子中发现,也是首次从药用植物红蓼中发现。结论该研究可为水红花子的深入质量评价及药效物质基础研究提供依据。Objective To establish a rapid identification method for the chemical components in the fructus of Polygounum orientale.Methods The MS1 and MS2 spectral data collected in both positive and negative ion modes was acquired with UPLC-Q-Orbitrap-MS.The constituents were identified by deducing the fragmentation patterns and comparing with mass databases(MassBank,mzCloud),literature data,and reference materials.Results A total of 54 chemical components were identified from the fructus of P.orientale,mainly including flavonoids,phenolic acids,alkaloids,etc.Among them,15 components were first discovered from the fructus of P.orientale and also from the whole plant of P.orientale.Conclusion This study can provide a basis for in-depth quality evaluation and pharmacological substance basis research for the fructus of P.orientale.

关 键 词：水红花子 化学成分 超高效液相串联四极杆静电场轨道阱质谱 质谱裂解规律 

分 类 号：R284.1[医药卫生—中药学]