基于网络药理学探讨艾草抗鸡马立克病毒的活性成分与作用机制  

Investigation on Anti‐MDV Active Components and Mechanism of Artemisia argyi Lévl.et Van.Based on Network Pharmacology

作　　者：马圣明 张福良 翁少亭 邢月腾 王尧 张坤朋 MA Shengming;ZHANG Fuliang;WENG Shaoting;XING Yueteng;WANG Yao;ZHANG Kunpeng(Henan Joint International Research Laboratory of Veterinary Biologics Research and Application,Anyang Institute of Technology,Anyang 455000,China)

机构地区：[1]安阳工学院河南省兽用生物制品研发与应用国际联合实验室,河南安阳455000

出　　处：《河南农业科学》2024年第3期133-140,共8页Journal of Henan Agricultural Sciences

基　　金：河南省重点研发与推广专项(222102110290);河南省高等学校重点科研项目(23B310006);安阳市重大科技专项(2021A01NY004);安阳工学院博士启动资金项目(BSJ2021007)。

摘　　要：运用网络药理学方法探讨艾草抗鸡马立克病毒(Marek’s disease virus,MDV)的活性成分、作用靶点及可能的作用机制。利用TCMSP数据库检索艾草化学成分和作用靶点,查阅文献收集MDV相关宿主基因靶点,建立靶点数据库;利用STRING数据库结合Cytoscape 3.8.0软件构建靶点蛋白相互作用网络及活性成分-关键靶点网络,筛选核心靶点和主要活性成分;利用DAVID数据库对关键靶点进行GO功能分析和KEGG通路富集分析;最后利用分子对接技术验证主要活性成分与关键靶点的结合能力。结果显示,从艾草中筛选出8种活性成分,共有108个基因靶点与MDV宿主基因存在相互作用,艾草抗MDV作用的主要靶点有JUN、CCND1、CDK1、IL-6等;GO功能分析涉及细胞活性、炎症反应等过程,KEGG富集分析涉及C型凝集素受体信号通路、MAPK信号通路、细胞衰老、p53信号通路、细胞凋亡、Toll样受体信号通路等;异泽兰黄素与靶点蛋白CCND1的对接能力最强。结果表明,艾草主要活性成分异泽兰黄素可能是通过作用于CCND1,干预细胞周期影响细胞活性,进而发挥抗MDV效应。The purpose of this study was to explore the active components,targets and possible mechanisms of Artemisia argyi Lévl.et Van.against Marek’s disease virus(MDV)by using network pharmacological methods.The TCMSP database was used to search the chemical components and action targets of Artemisia argyi Lévl.et Van.,and the MDV related host gene targets were collected through relevant literature to establish a target database;by using STRING database and Cytoscape 3.8.0 software,target protein interaction network and active ingredient‐key target network were constructed to screen core targets and main active ingredients;the DAVID database was used to analyze GO function and KEGG pathway enrichment of key targets;finally,molecular docking technology was used to verify the binding ability of the main active ingredients to key targets.The results showed that eight active components were screened from Artemisia argyi Lévl.et Van.,and 108 gene targets interacted with MDV host genes.The main targets of Artemisia argyi Lévl.et Van.with anti‐MDV action were JUN,CCND1,CDK1,IL‐6,etc;GO analysis involved cell activity,inflammatory reaction and other processes,and KEGG enrichment analysis involved C‐type lectin receptor signal pathway,MAPK signal pathway,cell aging,p53 signal pathway,apoptosis,Toll like receptor signal pathway,etc.The molecular docking results showed that the binding ability of eupatilin to the target protein CCND1 was the strongest.The results indicate that the main active component of Artemisia argyi Lévl.et Van.,eupatilin,may interfere with cell cycle and affect cell activity by acting on CCND1,thereby exerting anti‐MDV effects.

关 键 词：艾草 网络药理学 马立克病毒 活性成分 靶点 

分 类 号：S852.65[农业科学—基础兽医学]