毛细管离心技术在RhD抗原混合外观患者D抗原鉴定中的应用  

Capillary Centrifugation Technology for RhD Typing in Patients with Mixed Field Reactivity

作　　者：陈吉翠 高海燕[1] 马现君[1] CHEN Jicui;GAO Haiyan;MA Xianjun(Department of Blood Transfusion,Qilu Hospital of Shandong University,Jinan,Shandong,250012)

机构地区：[1]山东大学齐鲁医院,山东济南250012

出　　处：《临床输血与检验》2024年第2期236-239,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：山东省自然科学基金(No.ZR2020QH087)资助。

摘　　要：目的探讨毛细管离心技术在RhD抗原呈混合外观患者D抗原鉴定中的应用可行性。方法对11例RhD抗原呈混合视野的患者红细胞进行清洗,制备压积红细胞。之后用毛细管离心法分离血细胞,分别向近端患者自身红细胞和远端红细胞加入抗D试剂鉴定RhD抗原。根据献血者代码追踪输入红细胞的RhD抗原。结果经毛细管离心,1例患者RhD抗原阴性,8例患者RhD抗原阳性,其余两例患者RhD抗原仍为混合视野。结论毛细管离心技术可有效分离患者新生红细胞,准确鉴定患者RhD抗原,为临床输血提供安全保障。Objective To discuss the feasibility of the application of capillary centrifugation technology in RhD typing in patients with mixed field reactivity.Methods The red blood cells(RBCs)of 11 patients with mixed field reactivity in RhD typing were washed for hematocrit cells.The blood cells were separated by capillary centrifugation technology and the D antigen was identified by adding anti-D reagents into the proximal and distal red blood cells.The D antigen of transfused RBCs was traced against the blood donor code.Results By capillary centrifugation,RBCs showed 1 RhD-negative,8 RhDpositives and 2 mixed field reactivities.Conclusion Capillary centrifugation technology can effectively separate the new RBCs to identify the D antigen to provide blood transfusion safety.

关 键 词：毛细管离心技术 RHD抗原 血型鉴定 

分 类 号：R457[医药卫生—治疗学]