毛白杨的组织培养  

Tissue Culture of Populus tomentosa Carrière

作　　者：赵桂琴 何永芳 Zhao Guiqin;He Yongfang(State-owned Fengjiahe Forest Farm of Chencang District of Baoji City in Shaanxi Province,Baoji,Shaanxi 721300)

机构地区：[1]陕西省宝鸡市陈仓区国有冯家河林场,陕西宝鸡721300

出　　处：《中国林副特产》2024年第2期27-30,共4页Forest By-product and Speciality in China

摘　　要：以毛白杨试管苗带芽茎段为外植体,以MS为基本培养基,附加不同浓度的6-BA或IBA和不同浓度的NAA。分析不同浓度的6-BA、IBA和NAA分别对外植体启动、增殖和生根的影响,以及不同激素组合分别对外植体启动、增殖和生根的影响,探究最佳的启动培养基、分化培养基和生根培养基。结果表明:启动培养基以MS+6-BA0.5 mg/L+NAA 0.1 mg/L为最佳启动培养基;生根培养基以MS+IBA0.2 mg/L+NAA 0.1 mg/L为最佳生根培养基;分化培养基以MS+6-BA1.0 mg/L+NAA 0.1 mg/L为最佳分化培养基,但与理论结果不符,需要进一步实验研究。This study select stem segments with buds of as explants,MS as the basic medium with 6-BA or IBA and NAA of different concentration.Analysis the effects on explants’starting,proliferation,rooting with different concentration of 6-BA、IBA and NAA,and the effect of different hormone combination on the starting,proliferation,rooting of explants,exploration the best initial medium,differentiation medium and rooting medium.The result shows:The best rooting medium is MS+IBA0.2 mg/L+NAA0.1 mg/L.The best differentiation medium is MS+6-BA1.0 mg/L+NAA0.1 mg/L.The best initial medium is MS+6-BA0.5 mg/L+NAA0.1 mg/L.But the result above do not match the theory that reported,needs studying further.

关 键 词：毛白杨 组织培养 生根培养基 分化培养基 启动培养基 

分 类 号：S792.117[农业科学—林木遗传育种]