新冠疫情大流行期间广州市H3N2流感病毒分子流行特征分析  

Analysis of molecular epidemic characteristics of H3N2 influenza viruses in Guangzhou City during the COVID-19 pandemic

作　　者：曹蓝[1] 周腾飞 陈艺韵[1] 马蒙蒙 夏丹[1] 刘艳慧[1] 李魁彪[1] 狄飚[1] 秦鹏哲[3] 张周斌[3] Cao Lan;Zhou Tengfei;Chen Yiyun;Ma Mengmeng;Xia Dan;Liu Yanhui;Li Kuibiao;Di Biao;Qin Pengzhe;Zhang Zhoubin(Virus Immunology Department,Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,China;Department of Infectious Disease Prevention and Control,Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,China;Director′s Office,Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,China)

机构地区：[1]广州市疾病预防控制中心病毒免疫部,广州510440 [2]广州市疾病预防控制中心传染病预防控制部,广州510440 [3]广州市疾病预防控制中心主任室,广州510440

出　　处：《中华预防医学杂志》2024年第3期351-357,共7页Chinese Journal of Preventive Medicine

基　　金：广州市科技计划项目(202201010794、2023A03J0929);广州市医学重点学科(2021-2023-11)。

摘　　要：分析新型冠状病毒疫情(新冠疫情)大流行期间广州市H3N2流感病毒分子变异特征。收集流感监测哨点医院流感样病例样本,进行病毒分离和全基因组高通量测序。结果显示,新冠疫情大流行期间广州市在2022年第二季度出现了一次H3N2流感流行高峰(阳性率为52.23%),流行强度和持续时间均高于2019年H3N2流感流行期。2022年流行株HA基因和NA基因均属于3C.2a1b.2a.1a.1分支,与2019年冬季的H3N2流行株进化分支(Group-3 毒株3C.2a1b.1a)不同。2022年流行株抗原位点变异主要发生在C区I48T位,HA蛋白受体结合位点与疫苗株一致。大部分流行株在HA蛋白上带有13个糖基化位点,一起暴发疫情分离株发生24-NST处糖基化位点的缺失。综上,新冠疫情大流行期间,引起广州市2022年H3N2流感流行的病毒株属于新衍生进化分支,进化起源上并非由2019年广州市毒株直接传播进化而来。To monitor and analyze the molecular variation of the H3N2 influenza virus in Guangzhou during the COVID-19 pandemic,respiratory samples of influenza-like cases from influenza monitoring sentinel hospitals were collected from influenza monitoring sentinel hospitals for virus isolation and whole genome sequencing.The results showed that during COVID-19,there was only one peak of H3N2 influenza in the second quarter of 2022 in Guangzhou(the positive rate was 52.23%),and the epidemic intensity and duration were both higher than those in 2019.The HA gene and NA gene of the epidemic strain in Guangzhou in 2022 belonged to the 3C.2a1b.2a.1a.1 branch,which had a good antigenic site matching with the vaccine strain(A/Cambodia/e0826360/2020)from 2021 to 2022 and had no antigen drift.In 2022 strains,the variation of antigen determinant mainly occurred in the I48T of C region,while no variation occurred in the A,B,D,and E regions.The binding site of the HA protein receptor was consistent with the vaccine strain(A/Cambodia/e0826360/2020).Most of the strains in 2022 carried 13 glycosylation sites on the HA protein,but an outbreak of strains caused a loss of glycosylation sites at 24-NST.In conclusion,the strains that caused the epidemic of H3N2 influenza in Guangzhou in 2022 were not evolved or transmitted from the local strains in 2019 during the COVID-19 pandemic.

关 键 词：H3N2流感病毒 分子变异 遗传特征 新型冠状病毒 监测 

分 类 号：R511[医药卫生—内科学]