银钟花根尖染色体制片及核型分析  

Optimization of Chromosome Preparation Technology and Karyotype Analysis of Perkinsiodendron macgregorii(Chun)P.W.Fritsch

作　　者：闫明慧 孙文光[2] 张银 宁汪洋 刘彬文 YAN Minghui;SUN Wenguang;ZHANG Yin;NING Wangyang;LIU Binwen(College of Life Sciences,Xinyang Normal University,Xinyang 464000,China;College of Life Sciences,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]信阳师范大学生命科学学院,河南信阳464000 [2]云南师范大学生命科学学院,云南昆明650500

出　　处：《信阳师范学院学报（自然科学版）》2024年第2期222-227,共6页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31800276);河南省高等学校重点科研项目(19A180029);信阳师范学院“南湖学者奖励计划”青年项目。

摘　　要：以银钟花种子的初生根尖为材料,探讨不同预处理试剂、预处理时间和解离方法对银钟花根尖染色体压片的影响,获得了一套银钟花染色体制片技术,并以此为基础进行核型分析,揭示其核型特征。结果表明:用0.003 mol/L的8-羟基喹啉避光处理6 h,卡诺氏固定液固定10 h,1 mol/L HCl在60℃的恒温水浴锅中解离12 min,20%的卡宝品红染色,可获得分散较好、形态清晰的高质量银钟花染色体压片。银钟花的染色体数目为2n=24,核型公式为2n=24=20m+4Sm,核型不对称系数为55.77%,核型分类属于1B型。The root tips of P.macgregorii seeds was used as the research material to investigate the effects of different pretreatment reagents,pretreatment time and dissociation methods on the chromosome preparation of P.macgregorii root tips.A set of chromosome preparation technique of P.macgregorii was obtained and used as the basis for karyotype analysis to reveal its karyotype characteristics,providing a foundation for the conservation and genetic research of P.macgregorii.The results showed that pretreatment with 0.003 mol/L 8-hydroxyquinoline in the dark for 6 h,fixation with Carnoy’s fixative for 10 h,dissociation with 1 mol/L HCl in a constant temperature water bath at 60℃for 12 min,and staining with 20%carbol fuchsin could obtain high-quality chromosome squash of P.macgregorii with good dispersion and clear morphology.The chromosome number of P.macgregorii was 2n=24,and the karyotype formula was 2n=24=20m+4Sm,with an asymmetry coefficient of 55.77%.The karyotype was classified as type 1B.

关 键 词：银钟花 染色体 制片技术 核型分析 根尖 

分 类 号：Q492.4[生物学—生理学]