淫羊藿苷对前列腺癌原位移植瘤小鼠肿瘤生长及细胞周期的影响  

Effects of Icariin on Tumor Growth and Cell Cycle in Prostate Cancer Orthotopic Transplantation Tumor Mice

作　　者：唐荣志[1] 赖海标[1] 黄智峰[1] 刘毅豪[1] 吴惠妃[1] TANG Rongzhi;LAI Haibiao;HUANG Zhifeng;LIU Yihao;WU Huifei(Department of Urology,Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,China)

机构地区：[1]中山市中医院泌尿外科,广东中山528400

出　　处：《中西医结合研究》2024年第2期78-82,共5页Research of Integrated Traditional Chinese and Western Medicine

基　　金：广东省中医药局科研项目(No.20222249)。

摘　　要：目的探讨淫羊藿苷对前列腺癌原位移植瘤SCID小鼠肿瘤生长、细胞周期、磷酸酶及张力蛋白同源物(phosphatase and tensin homolog,PTEN)基因表达的影响。方法40只雄性SCID小鼠,根据随机数字表法随机均分为模型对照组、低剂量组、中剂量组、高剂量组,每组10只。采用SCID小鼠前列腺腺体背外侧包膜内注射人前列腺癌LNCaP细胞株悬液的方法构建前列腺癌原位移植瘤小鼠模型。造模2周后,模型对照组予以0.9%氯化钠注射液,低剂量组予以10 mg/kg淫羊藿苷,中剂量组予以40 mg/kg淫羊藿苷,高剂量组予以80 mg/kg淫羊藿苷。灌胃处理1次/d,治疗时间为5周。在灌胃给药治疗前及治疗5周后,分别取各组小鼠5只,对比各组肿瘤质量、肿瘤体积、肿瘤抑制率。采用流式细胞学方法检测LNCaP细胞周期,计算各周期比值。采用实时定量PCR检测前列腺肿瘤组织中PTEN mRNA相对含量。结果治疗后,模型对照组肿瘤质量、肿瘤体积较治疗前明显增加(P<0.05);中剂量组及高剂量组肿瘤质量、肿瘤体积较治疗前明显降低(P<0.05),且显著低于模型对照组(P<0.05)。中剂量组及高剂量组肿瘤抑制率显著高于低剂量组(P<0.05)。中剂量组及高剂量组肿瘤细胞S期较治疗前明显增加(P<0.05),且显著高于模型对照组(P<0.05);中剂量组及高剂量组肿瘤细胞G_(0)/G_(1)期较治疗前明显减少(P<0.05),且显著低于模型对照组(P<0.05)。中剂量组及高剂量组PTEN mRNA相对含量较治疗前明显增加(P<0.05),且显著高于模型对照组(P<0.05)。结论淫羊藿苷可能通过上调PTEN表达、改变细胞周期分布来抑制前列腺癌LNCaP细胞增殖,从而有效抑制前列腺癌原位移植瘤SCID小鼠肿瘤生长。Objective To investigate the effects of icariin on tumor growth,cell cycle and expression of phosphatase and tensin homolog(PTEN)genes in SCID mice with prostate cancer orthotopic transplantation tumor.Methods Forty male SCID mice were randomly divided into model control group,low-dose group,medium-dose group and high-dose group according to the random number table method,with 10 mice in each group.Mouse model of prostate cancer orthotopic transplantation tumor was constructed by injecting human prostate cancer LNCaP cell line suspension into the dorsal lateral capsule of the prostate gland in SCID mice.After 2 weeks of modeling,the model control group was given 0.9%sodium chloride injection,the low-dose group was given 10 mg/kg icariin,the medium-dose group was given 40 mg/kg icariin,and the high-dose group was given 80 mg/kg icariin.Gavage treatment once a day for 5 weeks.Before and after 5 weeks of gavage treatment,5 mice in each group were taken and compared for tumor mass,tumor volume,and tumor inhibition rate.Flow cytometry was used to detect the cell cycle of LNCaP and calculate the ratio of each cycle.Real time quantitative PCR was used to detect the relative content of PTEN mRNA in prostate tumor tissue.Results After treatment,tumor mass and volume in the model control group were significantly increased compared to before treatment(P<0.05).Tumor mass and volume in the medium-dose and high-dose groups were significantly reduced compared to before treatment(P<0.05),and were significantly lower than those in the model control group(P<0.05).The tumor inhibition rates in the medium-dose and high-dose groups were significantly higher than those in the low-dose group(P<0.05).The S phase of tumor cells in the medium-dose and high-dose groups was significantly increased compared to before treatment(P<0.05),and was significantly higher than that in the model control group(P<0.05).The G_(0)/G_(1) phase of tumor cells in the medium-dose and high-dose groups was significantly reduced compared to before treatment(P<0.

关 键 词：淫羊藿苷 前列腺癌 磷酸酶及张力蛋白同源物 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R737.25[医药卫生—中医学]