Identification and virulence test of a new pathogen that causes verticillium striping on rapeseed in northwestern China  

作　　者：We Si Ruisheng Wang Mingde Wu Long Yang Guoqing Li Jing Zhang 

机构地区：[1]State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province,Huazhong Agricultural University,Wuhan 430070,China [2]Qinghai Academy of Agriculture and Forestry Sciences,Qinghai University,Xining,Qinghai Province,810016,China

出　　处：《Oil Crop Science》2024年第1期8-19,共12页中国油料作物学报(英文版）

基　　金：supported by the Earmarked Fund for CARS-12 from National Modern Agricultural Technology System.

摘　　要：Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province,China,and fungal isolates were obtained from the stems.They were identified based on morphology,molecular features and specific PCR detection.The results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/D3.One of the 10 isolates(HW7-1)was tested for virulence on three species of rapeseed,including B.napus Zhongshuang 9,B.rapa Qingyou 9 and B.juncea Tayou 2 by conidia inoculation of HW7-1 on roots of young seedlings.Control seedlings were inoculated with V.dahliae conidia or water alone.The seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber(20℃).Results suggested that the control seedlings of three cultivars appeared quite healthy,while the seedlings inoculated with HW7-1 turned yellowing leaves,seedling stunting or even death after 22 days post-inoculation.V.longisporum was re-isolated from he yellow leaves,thus fulfilling Koch's postulates.Moreover,compared to the control treatments,inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the stems.To our knowledge,this is the first report of V.longisporum lineage A1/D3 on rapeseed in northwestern China.

关 键 词：Verticillium longisporum lineage A1/D3 VIRULENCE RAPESEED China 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]