五味子乙素对心肌梗死后大鼠心肌细胞凋亡的影响  

Effects of Schisandrin B on myocardial cell apoptosis in rats after myocardial infarction

作　　者：黄艳冰 陈伟文[1] 黄燕龙 林天来[1] HUANG Yan-bing;CHEN Wei-wen;HUANG Yan-long;LIN Tian-lai(Department of Critical Care Medicine,Quanzhou First Hospital Afiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China;Department of Emergency,Quanzhou FirstHospital Afiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China)

机构地区：[1]福建医科大学附属泉州第一医院重症医学科,福建泉州362000 [2]福建医科大学附属泉州第一医院急诊科,福建泉州362000

出　　处：《中国临床药理学杂志》2024年第7期999-1003,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究五味子乙素对心肌梗死大鼠的保护作用及其作用机制。方法将SD大鼠随机分为假手术组、模型组、阳性对照组(10mg·kg^(-1)卡托普利)、低剂量实验组(30mg·kg^(-1)五味子乙素)、高剂量实验组(60mg·kg^(-1)五味子乙素),每组10只,连续给药14d后检测心功能相关指标。以试剂盒检测血清心肌损伤标志物及血清炎症因子水平,以原位末端标记(TUNEL)法和蛋白质印迹法分别检测大鼠心肌细胞凋亡和相关蛋白的表达水平。结果假手术组、模型组、阳性对照组、低剂量实验组、高剂量实验组大鼠的左心室射血分数(LVEF)水平分别为(78.42±4.32)%、(41.65±2.94)%、(59.76±5.35)%、(49.13±3.92)%和(67.04±3.00)%,肌酸激脢同工酶(CK-MB)含量分别为(33.95±2.68)、(100.51±3.92)、(48.27±3.70)(70.34±2.93)和(49.13±3.67)U·L^(-1),白细胞介素-1β(IL-1β)水平分别为(1.02±0.12)、(3.02±0.20)、(1.61±0.13)、(2.33±0.26)和(1.34±0.14)ng·mL^(-1),TUNEL阳性细胞率分别为(3.47±0.82)%、(31.79±3.68)%、(11.22±1.02)%、(19.74±1.42)%和(14.38±1.13)%,核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)蛋白相对表达水平分别为0.35±0.07、1.04±0.10、0.55±0.05、0.85±0.07和0.39±0.06,胱天蛋白酶-1(caspase-1)蛋白相对表达水平分别为0.34±0.05、1.05±0.10、0.50±0.06、0.72±0.05和0.46±0.03;模型组的上述指标与假手术组比较,阳性对照组、低剂量实验组、高剂量实验组的上述指标与模型组比较,高剂量实验组的上述指标与低剂量实验组比较,在统计学上差异均有统计学意义(均P<0.05)。结论五味子乙素可介导炎性反应和心肌细胞凋亡,改善心功能,对心肌梗死大鼠起到保护作用,与NLRP3炎性通路相关。Objective To investigate the protective effect of Schisandrin B on myocardial infarction in rats and its mechanism.Methods Fifty SD rats were randomly divided into sham group,model group,positive control group(10 mg·kg^(-1)c captopril),experimental-L group(30 mg·kg^(-1)schisandrin B)and experimental-H(60 mg·kg^(-1)schisandrin B)group,with 10 rats in each group.Cardiac function related indexes were detected,serum myocardial injury markers and serum inflammatory factors were detected by the kit,myocardial apoptosis was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)method,and myocardial tissue related protein expression levels were detected by Western blot method.Results Left ventricular ejection fraction(LVEF)levels in sham group,model group,positive control group,experimental-L group and experimental-H group were(78.42±4.32)%,(41.65±2.94)%,(59.76±5.35)%,(49.13±3.92)%and(67.04±3.00)%;the contents of creatine kinase-MB(CK-MB)were(33.95±2.68),(100.51±3.92),(48.27±3.70),(70.34±2.93)and(49.13±3.67)U·mL^(-1);interleukin-1β(IL-1β)levels were(1.02±0.12),(3.02±0.20),(1.61±0.13),(2.33±0.26)and(1.34±0.14)ng·mL^(-1);TUNEL positive cell rates were(3.47±0.82)%,(31.79±3.68)%,(11.22±1.02)%,,(19.74±1.42)%and(14.38±1.13)%;the expression levels of Nod-like receptor thermal protein domain associated protein 3(NLRP3)protein were 0.35±0.07,1.04±0.10,0.55±0.05,0.85±0.07 and 0.39±0.06;the protein levels of cysteine aspartic acid specific protease 1(caspase-1)were 0.34±0.05,1.05±0.10,0.50±0.06,0.72±0.05 and 0.46±0.03,respectively.The above indexes were compared between model group and sham group,the differences were statistically significant(all P<0.05).The positive control group,experimental-L group and experimental-H group were compared with model group,the differences were statistically significant(all P<0.05).The difference between experimental-H group and experimental-L group were statistically significant(all P<0.05).Conclusion Schisandrin B can mediate infla

关 键 词：五味子乙素 心肌梗死 心肌细胞 炎症反应 核苷酸结合寡聚化结构域样受体蛋白3炎性通路 

分 类 号：R28[医药卫生—中药学]