金纳米探针用于危化品过氧化氢的检测研究  

Gold nanoprobes for the detection of hydrogen peroxide in hazardous chemicals

作　　者：顾小钰 邹宇鑫 杨莉 赵楠楠 柴志华[1] GU Xiaoyu;ZOU Yuxin;YANG Li;ZHAO Nannan;CHAI Zhihua(School of Chamical Safety,North China Institute of Science and Technology,Yanjiao 065201,China)

机构地区：[1]华北科技学院化工安全学院,北京东燕郊065201

出　　处：《华北科技学院学报》2024年第2期49-53,共5页Journal of North China Institute of Science and Technology

基　　金：河北省物联网监控技术创新中心绩效后补助经费项目(21567693H);中央高校基本科研业务费资助(3142021009)。

摘　　要：本文构建一种新型FRET纳米探针,用于H_(2)O_(2)的检测。首先通过紫外可见分光光度计、透射电镜及纳米粒度仪证实多糖功能化金纳米粒子的成功构建。接着利用荧光光谱仪证实荧光剂3-(丹磺酰氨)甲氧基苯硼酸成功组装到金纳米粒子的表面,形成简单可靠的FRET纳米探针。最后,利用荧光光谱仪证实,该纳米探针对H_(2)O_(2)的有效检测。In this study,a novel FRET nanoprobe was developed for the detection of H_(2)O_(2).Firstly,the successful construction of polysaccharide functionalized gold nanoparticles was confirmed by UV-vis spectrophotometer,transmission electron microscope and nanoparticle size analyzer.Then,the successful conjugation of 3-(dansyl ammonia)methoxyphenylboronic acid onto the surface of gold nanoparticles was confirmed through fluorescence spectroscopy,resulting in the development of straightforward and dependable FRET nanoprobes.Finally,the effective detection of H_(2)O_(2)was confirmed through the utilization of a fluorescence spectrometer.

关 键 词：金纳米粒子 葡聚糖 动态共价键 荧光共振能量转移 荧光淬灭 

分 类 号：R84.2[医药卫生—航空、航天与航海医学] Q943[医药卫生—临床医学]