采用经心尖人工腱索植入装置建立二尖瓣反流动物模型  

Establishment of mitral regurgitation model by a transapical artificial chordae tendineae implantation device in swines

作　　者：钟丽珊 杨燕晨 黄燕莹[1,3] 汪珍忠 肖硕 方斗 王秋吉 谢琦宗 张旭升 吴海明 黄焕雷[1,6] ZHONG Lishan;YANG Yanchen;HUANG Yanying;WANG Zhenzhong;XIAO Shuo;FANG Dou;WANG Qiuji;XIE Qizong;ZHANG Xusheng;WU Haiming;HUANG Huanlei(Guangdong Cardiovascular Institute,Guangdong Provincial People's Hospital(Guangdong Academy of Medical Sciences),Southern Medical University,Guangzhou,510080,P.R.China;The First People’s Hospital of Foshan,Foshan,528000,Guangdong,P.R.China;Guangdong Cardiovascular Institute,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,510080,P.R.China;South China University of Technology,Guangzhou,510641,P.R.China;Southern Medical University,Guangzhou,510515,P.R.China;Department of Cardiac Surgery,Guangdong Provincial People's Hospital(Guangdong Academy of Medical Sciences),Southern Medical University,Guangzhou,510080,P.R.China;Hanxin Medical Technology(Shenzhen)Co.,Ltd.,Shenzhen,518109,P.R.China)

机构地区：[1]广东省心血管病研究所、南方医科大学附属广东省人民医院(广东省医学科学院),广州510080 [2]佛山市第一人民医院,广东佛山528000 [3]广东省心血管病研究所、广东省人民医院、广东省医学科学院,广州510080 [4]华南理工大学,广州510641 [5]南方医科大学,广州510515 [6]南方医科大学附属广东省人民医院心外科(广东省医学科学院),广州510080 [7]瀚芯医疗科技(深圳)有限公司,广东深圳518109

出　　处：《中国胸心血管外科临床杂志》2024年第4期570-575,共6页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金面上项目(82270373);广东省省级科技计划项目(2020B1111170011);广东省基础与应用基础研究基金重点项目(2019B1515120071);广州市卫生健康委员会项目(2023FTJCZ0011)。

摘　　要：目的采用经心尖人工腱索植入装置建立二尖瓣反流动物模型,并验证装置的稳定性及可靠性。方法以大白猪为实验动物(n=12),在经心表超声引导下,经心尖小戳口植入二尖瓣人工腱索,通过调整人工腱索长度牵拉瓣叶导致二尖瓣反流。在术前、术后即刻、术后1个月、3个月记录心脏超声指标。结果所有模型均建立成功,术中及随访期无死亡,术后即刻二尖瓣均为中度反流。与术前相比,随访3个月时二尖瓣反流程度、心脏各径线均不同程度增加(P<0.05)。结论非体外循环下,超声引导经心尖植入腱索牵拉二尖瓣瓣叶的方法可稳定可靠地建立二尖瓣反流动物模型。Objective To research the procedure for creating an animal model of mitral regurgitation by implanting a device through the apical artificial chordae tendineae,and to assess the stability and dependability of the device.Methods Twelve large white swines were employed in the experiments.Through a tiny hole in the apex of the heart,the artificial chordae tendineae of the mitral valve was inserted under the guidance of transcardiac ultrasonography.Before,immediately after,and one and three months after surgery,cardiac ultrasonography signs were noted.Results All models were successfully established.During the operation and the follow-up,no swines died.Immediately after surgery,the mitral valve experienced moderate regurgitation.Compared with preoperation,there was a variable increase in the amount of regurgitation and the values of heart diameters at a 3-month follow-up(P<0.05).Conclusion In off-pump,the technique of pulling the mitral valve leaflets with chordae tendineae implanted transapically under ultrasound guidance can stably and consistently create an animal model of mitral regurgitation.

关 键 词：二尖瓣反流 动物模型 经心尖 人工腱索植入装置 

分 类 号：R654.2[医药卫生—外科学] R-332[医药卫生—临床医学]