肠道微生物与失眠之间的因果关系——一项两样本孟德尔随机化分析  

Causal relationship between gut microbiota and insomnia:a two-sample Mendelian randomized analysis

作　　者：郑雅丹 李磊 李洋 夏翠宏 黄茂茂 胡月 李丹 胥章彧 胥方元 王剑雄 ZHENG Ya-dan;LI Lei;LI Yang;XIA Cui-hong;HUANG Mao-mao;HU Yue;LI Dan;XU Zhang-yu;XU Fang-yuan;WANG Jian-xiong(Department of Rehabilitation Medicine,The Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China;不详)

机构地区：[1]西南医科大学附属医院康复医学科,四川泸州646099 [2]西南医科大学康复医学系,四川泸州646099 [3]康复医学与工程泸州市重点实验室,四川泸州646099

出　　处：《现代预防医学》2024年第7期1153-1160,共8页Modern Preventive Medicine

基　　金：四川省科学技术厅社会发展项目(23ZDYF2793);泸州科学技术和人才工作局重点研发项目(22097);合江县人民医院-西南医科大学一般项目(2021HJXNYD11)。

摘　　要：目的利用两样本孟德尔随机化方法探讨肠道微生物与失眠之间的因果关系。方法使用了来自MiBioGen联盟进行的最大的全基因组关联研究荟萃分析的肠道微生物群的汇总统计数据。失眠的汇总统计数据来自全基因组关联研究GWAS:失眠测量。采用逆方差加权、MR-Egger、加权中位数、简单众数法和加权众数法来研究肠道微生物群与失眠之间的因果关系。通过一系列敏感性分析来评估结果的稳定性。结果MR分析显示柔膜菌纲(β=-0.86,OR=0.42,95%CI:0.25~0.71)和未知科(β=-0.86,OR=0.42,95%CI:0.25~0.71)对失眠具有保护作用。此外,本研究还发现了其他14个类群与失眠之间存在潜在因果关系(P<0.05),分别为草酸杆菌科(F=170.153,P=0.034)、马文布赖恩特菌属(F=162.702,P=0.006)、瘤胃球菌UCG002属(F=298.323,P=0.046)、罕见小球菌属(F=198.442,P=0.045)、厌氧棒状菌属(F=222.074,P=0.032)、伽玛变形菌纲(F=91.816,P=0.006)、巴斯德菌目(F=231.141,P=0.010)、巴斯德菌科(F=231.141,P=0.010)、毛螺菌科属(F=138.761,P=0.045)、消化球菌属(F=287.210,P=0.036)、活泼瘤胃球菌属(F=162.913,P=0.031)、多尔属(F=112.086,P=0.049)、解黄酮菌属(F=95.307,P=0.020)及未知属(F=89.189,P=0.001)。未检测到显著的异质性或多效性。结论孟德尔随机化结果显示柔膜菌纲、未知科和其他14个类群与失眠有因果关系。肠道微生物可能成为改善失眠的治疗方法之一。Objective To investigate the causal relationship between gut microbiota and insomnia by two-sample Mendelian randomization analysis.Methods The summary statistics of gut microbiota came from a meta-analysis of the largest genome-wide association study conducted by the MiBioGen Alliance.The summary statistics for insomnia came from the genome-wide association study of insomnia measurement.Inverse variance weighted,MR-Egger,weighted median,simple mode method,and weighted mode method were used to study the causal relationship between gut microbiota and insomnia.The stability of the results was evaluated by a series of sensitivity analyses.Results MR analysis showed that soft membrane Mollicutes RF9[β=-0.86,OR(95%CI):0.42(0.25-0.71)]and unknown family[β=-0.86,OR(95%CI):0.42(0.25-0.71)]had protective effect on insomnia.In addition,14 other groups were found to be potentially causally associated with insomnia in this study including Oxalobacteraceae(F=170.153,P=0.034),Marvinbryantia(F=162.702,P=0.006),Ruminococcaceae UCG002(F=298.323,P=0.046),Subdoligranulum(F=198.442,P=0.045),Anaerostipes(F=222.074,P=0.032),Gammaproteobacteria(F=91.816,P=0.006),Pasteurellales(F=231.141,P=0.010),Lachnospiraceae UCG010(F=138.761,P=0.045),Peptococcus(F=287.210,P=0.036),Ruminococcus gnavus group(F=162.913,P=0.031),Dorea(F=112.086,P=0.049),Flavonifractor(F=95.307,P=0.020),and unknown genus(F=89.189,P=0.001).No significant heterogeneity or pleiotropy was detected.Conclusion Mendelian randomization shows that the flexurales,unknown families,and 14 other taxa are causally related to insomnia.The gut microbiota may be one of the treatments to improve insomnia.

关 键 词：肠道微生物 失眠 孟德尔随机化 

分 类 号：R256.23[医药卫生—中医内科学]