微管剪切蛋白调控果蝇节律神经元的形态发育  

Microtubule severing proteins regulate the morphology of Drosophila circadian clock neurons

作　　者：王顺 刘志华 WANG Shun;LIU Zhihua(School of Life Sciences,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2024年第3期297-305,共9页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(81871121)资助。

摘　　要：Spastin、Katanin60和Fidgetin是3种重要的微管剪切蛋白,它们的突变会导致神经退行性疾病。然而,这些微管剪切蛋白如何调节神经元的形态和功能尚不明确。果蝇的昼夜节律行为主要受位于大脑腹外侧的4对神经元LNv的控制。LNv神经元具有简单的形态,并且其轴突末梢会随着昼夜节律而伸缩。本研究利用LNv神经元作为模型,探讨不同微管剪切蛋白在神经元形态发育中的功能差异。我们发现,在LNv神经元中组成性表达Spastin会导致轴突和树突的发育异常,并且抑制神经肽PDF的转运;在成虫时期特异性表达Spastin会引起神经纤维的退化。而在LNv神经元中过量表达Katanin60或Fidgetin则不会影响LNv神经元的形态和神经肽PDF的转运。我们的结果揭示了不同微管剪切蛋白在不同神经元中具有不同的功能特性。Spastin,Katanin60 and Fidgetin are three major microtubule-severing proteins,whose mutations cause neurodegenerative diseases.However,it is unclear how these microtubule-severing proteins regulate the morphology and function of neurons.The circadian rhythm behavior of Drosophila is mainly controlled by four pairs of neurons LNv located in the ventrolateral region of the brain.LNv neurons have a simple morphology,and their axon terminals expand and contract with the circadian rhythm.In this paper,we used LNv neurons as a model system to investigate the functional differences of different microtubule-severing proteins in neuronal morphogenesis.We found that constitutive expression of Spastin in LNv neurons leaded to abnormal development of axons and dendrites,and inhibited the transport of neuropeptide PDF;and the specific expression of Spastin in adult stage caused axonal degeneration.However,overexpression of Katanin60 or Fidgetin in LNv neurons did not affect the morphology and transport of neuropeptide PDF of LNv neurons.Our results reveal that different microtubule-severing proteins have different functional characteristics in different neurons.

关 键 词：果蝇 微管 微管剪切蛋白 神经元形态发育 昼夜节律 

分 类 号：Q421[生物学—神经生物学]