溶血磷脂酸对意大利蜜蜂进食的影响  

Effects of lysophosphatidic acid on the feeding of the Italian honey bee,Apis mellifera ligustica

作　　者：朱佳琦 杜开书[1] 杨萌[1] 张曌楠 ZHU Jia-Qi;DU Kai-Shu;YANG Meng;ZHANG Zhao-Nan(School of Resources and Environment,Henan Institute of Science and Technology,Xinxiang 453003,China;University of Toulouse,Toulouse 31062,France;School of Life Sciences,Peking University,Beijing 100871,China;Peking-Tsinghua Center for Life Sciences,Beijing 100871,China)

机构地区：[1]河南科技学院资源与环境学院,新乡453003 [2]University of Toulouse,Toulouse 31062,France [3]北京大学生命科学学院,北京100871 [4]北京大学-清华大学生命科学联合中心,北京100871

出　　处：《昆虫学报》2024年第3期358-365,共8页Acta Entomologica Sinica

基　　金：国家现代农业技术体系建设专项资金项目(CARS-44-SYZ9);北京大学-清华大学生命科学联合中心博士后基金。

摘　　要：【目的】探究肠道细菌代谢产物溶血磷脂酸(lysophosphatidic acid,LPA)对蜜蜂摄食行为的影响。【方法】对照组饲喂普通的蔗糖溶液,处理组饲喂含有LPA的蔗糖溶液,构建补充LPA的意大利蜜蜂Apis mellifera ligustica模型,利用群体水平取食测定法和个体水平取食测定法计算意大利蜜蜂6日龄成蜂的取食量,利用食物敏感度测定方法检测LPA对意大利蜜蜂成蜂食物敏感度的影响,最后对意大利蜜蜂成蜂的头、胸、腹和整个个体分别进行重量测定。【结果】LPA会减少意大利蜜蜂成蜂在群体水平上的取食量,对照组和处理组的群体水平每日平均取食量分别为4.23和2.38 mL。LPA不会影响意大利蜜蜂成蜂在群体水平上对食物的敏感度,也不会影响意大利蜜蜂成蜂在个体水平上对不适口食物的消耗量。分析不同饥饿水平意大利蜜蜂成蜂个体水平食物消耗量发现,LPA会使意大利蜜蜂成蜂个体水平上的饥饿感钝化,即使是饥饿状态下,处理组成蜂的取食量未能达到对照组的正常水平。对比意大利蜜蜂成蜂头、胸、腹和整个个体的重量发现,LPA降低成蜂的食物摄入导致头、胸、腹和整个个体重量显著下降。【结论】蜜蜂肠道细菌的代谢产物LPA会抑制宿主意大利蜜蜂成蜂的食物摄入,进而引起体重下降。【Aim】To explore the impact of the gut bacterial metabolite,lysophosphatidic acid(LPA)on food intake behavior in honey bees.【Methods】Fed with normal sucrose solution in the control group and sucrose solution containing LPA in the treatment group,a honey bee(Apis mellifera ligustica)model supplemented with LPA was established.The food intake of the 6-day-old adults of A.mellifera ligustica was measured using both group-level feeding assay and individual-level feeding assay.The sensitivity of A.mellifera ligustica adults to food was evaluated using a food sensitivity test.Additionally,the weight of the head,thorax,abdomen and whole individual of A.mellifera ligustica adults was measured.【Results】LPA reduced the food intake of A.mellifera ligustica adults at the group level.The group-level average daily food intake was 4.23 mL for the control group and 2.38 mL for the treatment group.LPA did not affect the food sensitivity of A.mellifera ligustica adults at the individual level or the intake of unpalatable food.The analysis of food intake at different hunger levels revealed that LPA blunted the hunger sensation in A.mellifera ligustica adults at the individual level.Even in a hungry state,the food intake of A.mellifera ligustica adults in the treatment group failed to reach the normal food intake level observed in the control group.Comparison of the weight of the head,thorax,abdomen,and whole individual of A.mellifera ligustica adults showed a significant decrease in weight due to reduced food intake caused by LPA.【Conclusion】LPA,the gut bacterial metabolite,inhibits food intake in the host A.mellifera ligustica adults,leading to a decrease in their body weight.

关 键 词：意大利蜜蜂 溶血磷脂酸 摄食行为 细菌 取食量 

分 类 号：Q966[生物学—昆虫学]