松墨天牛成虫卵巢发育过程及引诱剂诱集雌虫发育状态  

Ovarian developmental process of Monochamus alternatus(Coleoptera:Cerambycidae)adults and the developmental status of its females trapped by attractants

作　　者：朱浩诚 顾宇彤 郑凯文 董奕帆 樊建庭 ZHU Hao-Cheng;GU Yu-Tong;ZHENG Kai-Wen;DONG Yi-Fan;FAN Jian-Ting(National Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticides,College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学林业与生物技术学院,生物农药高效制备技术国家地方联合工程实验室,杭州311300

出　　处：《昆虫学报》2024年第3期384-392,共9页Acta Entomologica Sinica

基　　金：浙江省重点研发计划项目(2019C02023)。

摘　　要：【目的】探究松墨天牛Monochamus alternatus雌成虫卵巢发育过程,从日龄的角度对松墨天牛卵巢发育进行分级,并且判断引诱剂野外诱捕雌成虫的发育状态,为松墨天牛高效防控提供参考。【方法】对达到0, 5, 10, 15, 20, 30, 40和15日龄交配前后的松墨天牛雌成虫以及引诱剂诱集雌成虫进行生物解剖,观察卵巢发育状态。【结果】松墨天牛雌成虫卵巢发育过程分为5级:发育前期(0-5日龄):卵巢管透明纤细,无卵黄沉积;卵黄沉淀期(5-15日龄):卵巢管内含有少数乳白色和淡黄色未成熟的卵粒;卵巢成熟期(15-20日龄):卵巢管内包含大量完全成熟的卵粒,呈长纺锤形,黄色饱满有光泽;产卵盛期(20-40日龄):输卵管萼中堆满成熟卵粒,中输卵管和侧输卵管中存在待产的卵粒;衰老期(≥40日龄):卵巢管、输卵管萼和输卵管逐渐萎缩。交配后的雌成虫能够产生受精卵,输卵管萼膨大、形态清晰;未交配的雌成虫无法产生受精卵,卵粒堆积在输卵管中无法产出,输卵管萼未分化完全、形态不清晰。野外诱集结果显示,诱集的不同日龄的雌成虫量具有显著性差异,其中处于产卵盛期的雌成虫占比最多,占总量的54.90%±5.50%;卵巢成熟期、衰老期和卵黄沉淀期的雌成虫占比较少,分别占总量的31.37%±5.52%, 11.76%±1.54%和1.96%±0.51%;没有诱集到发育前期的雌成虫。【结论】松墨天牛雌成虫发育至15-20日龄达到性成熟;交配行为能够促进松墨天牛雌成虫卵巢发育。引诱剂可以诱捕到大量刚性成熟的松墨天牛成虫,对于减少松墨天牛产卵量,降低下一代发生基数,降低松材线虫Bursaphelenchus xylophilus传播几率具有积极意义。【Aim】To investigate the ovarian developmental process of female adults of Monochamus alternatus,categorize the ovarian development of M.alternatus from the perspective of age,and judge the developmental status of female adults trapped by attractants in the field,so as to provide a reference for effective prevention and control of M.alternatus.【Methods】The mated and unmated female adults of M.alternatus at the 0-,5-,10-,15-,20-,30-,40-and 15-day-old,and the female adults trapped by attractants were dissected to observe the ovarian development.【Results】The ovarian developmental process of female adults of M.alternatus can be divided into five stages:prophase(0-5-day-old):the ovariole is transparent and slender,and there is no egg yolk deposition;egg yolk precipitation stage(5-15-day-old):there are a few milky white and yellowish immature eggs in the ovariole;ovarian maturity stage(15-20-day-old):ovariole contains a large number of fully mature eggs,which are long spindle-shaped,yellow and full of luster;oviposition peak stage(20-40-day-old):mature eggs are piled up in the egg calyx,and there are eggs to be laid in the median oviduct and lateral oviduct;and aging stage(≥40-day-old):ovariole,egg calyx and oviduct gradually atrophy.After mating,the female adults can produce fertilized eggs,and the egg calyx is enlarged and visible.The unmated females can’t produce fertilized eggs,and the eggs can’t be produced when they are accumulated in the oviduct,and the egg calyx is not fully differentiated and the shape is invisible.The results of field trapping showed that there were significant differences in the number of trapped female adults at different day-old ages,and the proportion of the female adults in the oviposition peak stage was the most,accounting for 54.90%±5.50%of the total.The female adults at the ovarian maturity stage,aging stage and egg yolk precipitation stage were relatively few,accounting for 31.37%±5.52%,11.76%±1.54%and 1.96%±0.51%of the total,respectively.No female adults at the

关 键 词：松墨天牛 卵巢发育 日龄 引诱剂 诱集 

分 类 号：Q968[生物学—昆虫学]