梨树腐烂病病原菌种群结构分析  

Population structure analysis of pathogen of pear valsa canker in Aral area of Xinjiang

作　　者：唐丽 李春艳 孟紫微 李亚鹏[1] 张王斌[1] LI TANG;LI Chunyan;MENG Ziwei;LI Yapeng;ZHANG Wangbin(The National Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Tress in Southern Xinjiang/Key Lab of Xinjiang Production and Construction Corps in Comprehensive Agricultural Pest Management in Southern Xinjiang/Scientific Observing and Experimental Station of Crops Pests in Aral,Ministry of Agriculture and Rural Affairs/Tarim University,Aral Xinjiang 843300,China;Xinjiang Applied Vocational and Technical College,Kuitun Xinjiang 833200,China)

机构地区：[1]塔里木大学/南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室/南疆农业有害生物综合治理兵团重点实验室/农业农村部阿拉尔作物有害生物科学观测实验站,新疆阿拉尔843300 [2]新疆应用职业技术学院,新疆奎屯833200

出　　处：《新疆农业科学》2024年第3期681-689,共9页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(31660034)。

摘　　要：【目的】分析新疆生产建设兵团第一师阿拉尔市库尔勒香梨园梨树腐烂病病原菌的种群分类,通过6对保守基因序列,研究库尔勒香梨腐烂病病原菌序列之间相关性及差异性。【方法】在库尔勒香梨园内采集具有典型症状的腐烂病病样,经科赫氏法则验证获取腐烂病病原菌纯培养。采用CTAB法提取腐烂病病原菌基因组DNA,通过PCR扩增ITS、β-tubulin、EF-1α、ACT、LSU、RBP2基因并测序,利用分子系统学分析不同基因片段的系统发育关系。【结果】在阿拉尔周边混交园内库尔勒香梨腐烂病菌种为Valsa mali var.pyri(V.ambiens、Valsa mali)。种内差异与地理位置有关,国内与国外梨腐烂病分离株差异较明显。EF-1α、ACT、LSU、RBP2在进化过程中更能够体现种间差异,系统发育树分支多,存在种间差异。【结论】新疆生产建设兵团第一师阿拉尔市库尔勒香梨腐烂病病原菌种群分布和种间差异性,库尔勒香梨园的腐烂病病原菌主要为Valsa mali var.pyri、Valsa sordida。新疆南疆绿洲特色果园种植模式,以杨树等为主防护林最为常见,存在着林果树木腐烂病交叉侵染的可能。【Objective】The purpose of this study is to investigate the population classification and difference by 6 pairs of gene sequences of the pathogens from Aral City,Ist Oivision,Xinjiang Production and Constraction Corps.【Methods】Typical disease samples were collected for purified pathogens by Koch postulates.The total DNA of pathogens was extracted following a standard cetyl-trimethyl ammonium bromide(CTAB)method,subsequently,the ITS,β-tubulin,EF-1α,ACT,LSU,and RBP2 genes were conducted by PCR amplification and DNA sequencing.【Results】Pathogenic species of korla pear rot in mixed gardens around Alar Valsa mali var.pyri(V.ambiens、Valsa mali).Species differences are related ro geographical tocation.There are significant differences in pear rot disease isolates between domestic and foreign and foreign countries.EF-1α、ACT、LSU、RBP2 is more able to reflect interspecific differences,and phylogenetic tree has multiple branches,resulting in interspecific differences.【Conclusion】The results showed that Valsa mali var.pyri and Valsa sordida were the main diseases in Korla pear.It was clear that the difference existed between the species and population distribution of canker disease pathogen in Korl pear.The main shelterbelt such as poplar is the most common windbreak in Southern Xinjiang,maybe there is a cross infection of fruit trees and wood trees.

关 键 词：基因序列 Valsa mali var.pyri Valsa sordida 分子鉴定 

分 类 号：S436.611.11[农业科学—农业昆虫与害虫防治]