紫花苜蓿炭疽病病原新记录种(Colletotrichum liriopes)的鉴定及生物学特性分析  

Identification of new pathogens of alfalfa anthracnose(Colletotrichum liriopes)and preliminary study on biological characteristics

作　　者：柳丰 祖丽皮耶·安外尔 李克梅[1] 托伦巴特·毕亚洪 LIU Feng;Zulipiye Anwaier;LI Kemei;Tuolunbate Biyahong(College of Agronomy,Xinjiang Agricultural University/Key Laboratory of Prevention and Control of Invasive Organisms in Agriculture and Forestry in Northwest Desert Oases,Ministry of Agriculture and Rural Affairs(co-constructed by Ministry and Province)/Key Laboratory of Monitoring and Safety Prevention and Control of Agricultural and Forestry Pests,Urumqi 830052,China;Agriculture(animal husbandry)development center,Ergong town,Tacheng,Tacheng Xinjiang 834300,China)

机构地区：[1]新疆农业大学农学院/农业农村部西北荒漠绿洲农林外来入侵生物防控重点实验室(部省共建)/农林有害生物监测与安全防控重点实验室,乌鲁木齐830052 [2]塔城地区塔城市二工镇农业(畜牧业)发展中心,新疆塔城834300

出　　处：《新疆农业科学》2024年第3期690-698,共9页Xinjiang Agricultural Sciences

基　　金：国家重点研发计划项目(2022YFD1401101)。

摘　　要：【目的】从炭疽病症状的紫花苜蓿病株分离得到一种炭疽菌,与此前报道的三叶草炭疽菌(Colletotrichum trifolii)、北美炭疽菌(C.americae-borealis)形态上略有差异,研究该菌致病性、分类地位及其生物学特性并鉴定。【方法】采用组织分离法获得炭疽菌菌株,经柯赫氏法则证明其致病性,并运用形态学观察结合多基因片段PCR扩增法鉴定病原菌种类,测定不同培养基、碳源、氮源、温度、pH值、光照对该菌菌落生长的影响。【结果】从紫花苜蓿炭疽病病部分离获得一种新的致病炭疽菌,为麦冬炭疽菌(Colletotrichum liriopes)。该菌在5~35℃,pH值4~11的多数供试培养基、氮源、碳源条件下均能生长。【结论】麦冬炭疽菌(C.liriopes)可侵染紫花苜蓿引起炭疽病,其菌落最适生长温度为28℃,最佳氮源、碳源分别是酵母和可溶性淀粉,最适pH为7,菌丝致死温度61℃,12 h光暗交替对菌落生长有利。【Objective】This project aims to isolate an anthrax strain from alfalfa with the symptoms of anthracnose,which would be slightly different from the previously reported Colletotrichum trifolii and C.americae borealis in morphology in order to clarify the pathogenicity,taxonomic status and biological characteristics of this strain through a series of studies.【Methods】Anthrax strain was obtained by tissue isolation,and its pathogenicity was proved by Koch's rule and morphological observation combined with PCR amplification of polygene fragments was used to identify the pathogenic bacteria.Meanwhile,the effects of different culture media,carbon source,nitrogen source,temperature,pH value and light on the colony growth of anthrax strain were determined.【Results】A new pathogenic anthrax strain was isolated from alfalfa,and identified as Colletotrichum liriopes.The strain could grow under the conditions of 5-35℃and pH 4-11,and most of the test medium,nitrogen source and carbon source conditions.【Conclusion】C.liriopes can infect alfalfa and cause anthracnose.The optimum growth temperature of C.liriopes is 28℃,the optimum nitrogen source and carbon source are yeast and soluble starch respectively,the optimum pH is 7,the lethal temperature of mycelium is 61℃,and the alternation of light and dark for 12 h is favorable to the growth of the colony.

关 键 词：苜蓿炭疽病 麦冬炭疽菌 鉴定 生物学特性 

分 类 号：S435.5[农业科学—农业昆虫与害虫防治]