不同基因编辑类型对FGF5基因编辑羊羊毛性状的影响  

Effects of different gene editing types on traits of FGF5 gene-edited sheep wool

作　　者：岳成广 李忠慧 刘晨曦 贺三刚 马海叶 刘璇 李婧平 李文蓉 YUE Chenggung;LI Zhonghui;LIU Chenxi;HE Sangang;MA Haiye;LIU Xuan;LI Jingping;LI Wenrong(Institute of Biotechnology,Xinjiang Academy of Animal Sciences,Urumqi 830011,China;Key Laboratory of Animal Biotechnology of Xinjiang,Urumqi 830011,China;College of Animal Sciences,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆畜牧科学院生物技术研究所,乌鲁木齐830011 [2]新疆维吾尔自治区动物生物技术重点实验室,乌鲁木齐830011 [3]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2024年第3期734-741,共8页Xinjiang Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项子课题“长毛型转基因超细毛羊的培育”(2016ZX08008001-002)。

摘　　要：【目的】分析不同基因编辑类型对成纤维生长因子5(FGF5)基因编辑细毛羊羊毛性状的影响,为开展基因编辑细毛羊的羊毛性状遗传参数评估提供理论依据。【方法】收集181只FGF5周岁基因羊,分析编辑细毛羊羊毛自然长度、伸直长度、羊毛纤维直径和剪毛量等性状的数据。【结果】FGF5基因编辑细毛羊羊毛自然长度平均值为10.49 cm,变异系数为11.82%。FGF5-InDel和FGF5-HDR基因编辑细毛羊羊毛自然长度和剪毛量均极显著高于野生型(P<0.01)。-28 bp或-26 bp、2 bp和置换(c.61 C>T)的羊毛自然长度和伸直长度均极显著长于wt(P<0.01),-28 bp或-26 bp的剪毛量显著高于wt(P<0.05)。-28 bp基因型的双等位基因编辑和单等位基因编辑突变的羊毛自然长度和伸直长度均极显著长于野生型(P<0.01)。【结论】不同基因编辑类型的FGF5基因编辑细毛羊均可显著提高羊毛自然长度和剪毛量,可利用表型性状突出的后代组建核心编辑羊群体,促进FGF5基因编辑细毛羊选育扩繁。【Objective】To study the effects of different gene editing types on wool traits in FGF5 gene-edited fine-wool sheep in the hope of providing a theoretical basis for subsequent studies on the genetic parameters of wool traits in gene-edited fine-wool sheep.【Methods】In this study,data on traits such as wool length,wool straight length,wool fiber diameter and greasy fleece weight from 181 FGF5-year-old gene-edited fine-wood sheep from the sheep breeding base of Xinjiang Academy of Animal Sciences were collected for analysis.【Results】The wool length of FGF5 gene editing fine wool sheep was 10.49 cm with a coefficient variation of 11.82%.Wool length and greasy fleece weight of FGF5-InDel and FGF5-HDR gene-edited fine-wool were extremely significant higher than that of the wild type(P<0.01).The wool length and wool straight length of-28 bp or-26 bp,-2 bp and replacement(c.61 C>T)were extremely significant longer than that of the wild type(P<0.01),and the greasy fleece weight at-28 bp or-26 bp was significantly higher than that of the wild type(P<0.05).The wool length and wool straight length of double-allele-edited and single-allele-edited mutations of the-28 bp genotype had extremely significantly longer than those of thewild type(P<0.01).【Conclusion】Different gene editing types of FGF5 gene editing in fine wool sheep significantly increase the wool length and greasy fleece weight,which can be used to form a core population of gene-edited sheep with outstanding phenotypic traits,promote the selection and expansion of FGF5 gene-edited sheep.

关 键 词：FGF5基因编辑细毛羊 编辑类型 羊毛自然长度 剪毛量 基因型 突变类型 

分 类 号：S826[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]