异氟烷麻醉对小鼠自发肌电及TUS/TMAS诱发肌电的影响  

Effects of Isoflurane Anesthesia on Spontaneous and TUS/TMAS Induced Electromyography in Mice

作　　者：王茹茹 周晓青[1] 赵渝卉 刘煦 刘志朋[1] 王欣[1] 殷涛[1] Wang Ruru;Zhou Xiaoqing;Zhao Yuhui;Liu Xu;Liu Zhipeng;Wang Xin;Yin Tao(Institute of Biomedical Engineering,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China)

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192

出　　处：《中国生物医学工程学报》2024年第1期10-17,共8页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(81927806,52107241);中国医学科学院医学与健康科技创新工程(2021-I2M-1-058)。

摘　　要：经颅超声刺激(TUS)和经颅磁声耦合刺激(TMAS)调控运动皮层效果明显,但受限于清醒状态动物难以束缚,已有研究大多在麻醉状态下进行,对麻醉减弱调控效果的分析集中于中枢神经系统。本研究记录了异氟烷麻醉下24只小鼠的肢体自发肌电和TUS/TMAS诱发肌电,定量分析了麻醉对自发肌电和诱发肌电发放率、潜伏期、时长和幅值的影响。结果显示,随着异氟烷输出浓度从0.40%增加至0.75%,每周期内小鼠自发肌电频次减少约50%,肌电发放时长变短,呈抑制状态;TUS/TMAS诱发肌电的成功率分别降低约50%和70%、潜伏期均延长约0.1 s、时长分别缩短约0.3和0.5 s,表明TUS/TMAS对运动皮层的调控效果随麻醉程度的加深而减弱。肢体自发和诱发肌电在发放率和时长上存在关联性特征,提示麻醉状态下小鼠自发肌电抑制状态可能是刺激效果减弱的影响因素之一。Transcranial ultrasound stimulation(TUS)and transcranial magneto-acoustic stimulation(TMAS)are effective in modulating motor cortex.Previous studies have mostly been conducted under anesthesia confined to the difficulty of tethering conscious animals,and the analysis of the attenuating modulatory effects of anesthesia has primarily focused on the central nervous system.In this study,spontaneous electromyography(EMG)and TUS/TMAS-induced EMG were recorded during isoflurane anesthesia in 24 mice,the effects of anaesthetize on spontaneous and induced EMG were analyzed quantitatively in terms of firing rate,latency,duration and amplitude.Experimental results showed that the frequency of spontaneous EMG decreased by 50%per cycle approximately and the duration decreased,as the concentration of isoflurane increased from 0.4%to 0.75%,indicating an inhibitory state.The induced-EMG success rates of TUS/TMAS both decreased by 50%and 70%respectively,with an average increase of 0.1 s in latency and a decrease of 0.3 s and 0.5 s in duration respectively,suggesting that the modulatory effect of TUS/TMAS on the motor cortex attenuated as the depth of anesthesia increased.According to the observed correlation between the firing rate and duration of spontaneous and induced EMG,it is rational to infer that the suppression of spontaneous EMG in mice under anesthesia was one of the factors contributing to the attenuated modulatory effect.

关 键 词：经颅超声刺激(TUS) 经颅磁声耦合刺激(TMAS) 肌电 麻醉 

分 类 号：R318[医药卫生—生物医学工程]