火龙果响应盐旱胁迫转录组分析  

Transcriptome Analysis of Dragon Fruit in Response to Salt and Drought Stresses

作　　者：李俊成 戴宏芬[1] 孙清明[1] Li Juncheng;Dai Hongfen;Sun Qingming(Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization(Ministry of Agriculture and Rural Affairs),Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Institute of Fruit Tree Research,Guangdong Academy of Agricultural Science,Guangzhou,510640)

机构地区：[1]广东省农业科学院果树研究所,农业农村部南亚热带果树生物学与遗传资源利用重点实验室,广东省热带亚热带果树研究重点实验室,广州510640

出　　处：《分子植物育种》2024年第8期2518-2527,共10页Molecular Plant Breeding

基　　金：广东省农业科学院院长基金(2022);广东省重点领域研发计划项目(2018B020202011);广东省农业农村厅项目(2020KJ257);广东省现代农业产业技术体系优稀水果创新团队建设项目(2019KJ116);广东省乡村振兴战略专项(粤农计[2018]37号,粤财农[2019]73号)共同资助。

摘　　要：盐旱胁迫是植物生长过程中的常见威胁,且两种逆境经常同时出现,对植物生长和发育造成极大伤害。生理学研究认为盐旱逆境都会导致细胞渗透压改变,造成膜损伤等,产生的表型及生理变化相似,但是针对盐旱胁迫在基因表达调控层面差异的研究还很少。本研究分别使用PEG-6000及NaCl处理7日龄火龙果(Hylocereus spp.)幼苗,通过转录组测序及生物信息分析研究盐旱胁迫下基因表达变化,发现两种逆境胁迫下部分基因的表达模式是相似的,存在共有的调控通路和信号,但是也发现有些基因响应方式在两种胁迫下完全相反,大部分差异表达基因完全不同,说明火龙果应对盐旱胁迫的基因表达调控存在不同和相似之处。本研究筛选出大量同时响应两种胁迫的基因,可为未来选育具广谱抗性的火龙果品种提供理论依据。Salinity and drought stresses are frequent threats to plants throughout their lives,and the two frequently coexist,wreaking havoc on plant growth and development.According to physiological research,salinity and drought stresses cause changes in osmotic pressure,resulting in membrane damage and other effects.Similar phenotypic and physiological changes occur under salinity and drought stresses.However,few research have examined the differences or similarities between salinity and drought-induced gene expression regulation.This study uses PEG-6000and NaCl to treat seven days dragon fruit(Hylocereus spp.) seedlings.After analyzing the changes in gene expression caused by two different stresses using transcriptomic sequencing,it was discovered that while some genes expressed similarly under both stresses and there were some shared regulatory pathways and signals,it was also discovered that some genes responded in opposite patterns,with the majority of differentially expressed genes being completely different.This work identified a high number of genes that respond to both pressures simultaneously,establishing a theoretical foundation for future development of broad-spectrum resistant dragon fruit cultivars.

关 键 词：火龙果 干旱胁迫 盐胁迫 转录组 

分 类 号：S667.9[农业科学—果树学]