不同居群蒙古扁桃的遗传多样性SRAP分析  

SRAP Analysis of Genetic Diversity in Different Populations of Amygdalus mongolica

作　　者：丁海麦[1] 白羽 周红兵[1] 石松利[1] Ding Haimai;Bai Yu;Zhou Hongbing;Shi Songli(Baotou Medical College,Inner Mongolia University of Science&Technology,Baotou,014040)

机构地区：[1]内蒙古科技大学包头医学院,包头014040

出　　处：《分子植物育种》2024年第8期2583-2589,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(81641137,81760782);内蒙古自治区第十一批“草原英才”人才工程[(2021)8];内蒙古自然科学基金项目(2015MS0808,2019MS08189);内蒙古自治区高等学校科学研究项目(NJZY21048)共同资助。

摘　　要：本研究选取5个具有代表性野生蒙古扁桃种群为研究对象,以同科近属蒙古柄扁桃种群为对照,采用SRAP分子标记方法,对珍稀濒危药用植物蒙古扁桃遗传多样性进行研究,为蒙古扁桃资源的合理保护和可持续利用提供科学的依据。用筛选出的12对SRAP引物,通过优化的反应体系对60个样品进行扩增,共扩增出171个清晰可重复的条带,其中多态性条带有166条,占97.08%。在物种水平上,Nei’s基因多样度(h)0.371 1、Shannon信息指数(I)为0.546 8;种群间遗传分化系数(Gst)为0.596 4。结果显示蒙古扁桃居群的遗传变异主要存在于居群间,居群间的遗传分化程度大于居群内的,综合评价贺兰山的蒙古扁桃遗传多样性较高,应当优先保护。In this study,five different populations of Amygdalus mongolica were taken as the material research object,and the population of Prunus mongolica with the same genus and related species as the control.The genetic diversity of rare and endangered medicinal plant Amygdalus mongolica was studied by SRAP molecular marker method,so as to provide a scientific basis for the rational protection and sustainable utilization of Amygdalus mongolica resources.Using 12 pairs of SRAP primers screened,60 samples were amplified through the optimized reaction system.A total of 171 clear and repeatable bands were amplified,of which 166 were polymorphic,accounting for 97.08%.At the species level,Nei's gene diversity(h) was 0.371 1 and Shannon information index(I) was 0.546 8;The coefficient of genetic differentiation(Gst) among populations was 0.596 4.The results showed that the genetic variation of Amygdalus mongolica population mainly existed among populations,and the degree of genetic differentiation among populations was greater than that within populations.Through comprehensive evaluation,norikong had high genetic diversity and should be protected first.

关 键 词：蒙古扁桃 柄扁桃 SRAP 遗传多样性 

分 类 号：S662.9[农业科学—果树学]