4株滇重楼内生真菌诱导甾体皂苷合成机制  

The Mechanism of Four Endophytic Fungi in Inducing the Synthesis of Saponins of Pairs polyphylla var.yunnanensis

作　　者：任锡跃 李艳冰 陈严平[1] 斯那永宗 字淑慧[1] 刘涛[1] Ren Xiyue;Li Yanbing;Chen Yanping;Si Nayongzong;Zi Shuhui;Liu Tao(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,650201;Diqing Tibetan Autonomous Prefecture Deqin County Agricultural Comprehensive Service Center,Diqing,674499)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]迪庆州德钦县农业综合服务中心,迪庆674499

出　　处：《分子植物育种》2024年第8期2762-2771,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31560085)资助。

摘　　要：内生真菌能通过代谢促进植物生长,还能够刺激药用植物中有用成分的积累。针对滇重楼生长缓慢,药用成分积累所需周期长等问题,本研究选用4株内生真菌并接种滇重楼幼苗,检测宿主植物学性状、光合特性、甾体皂苷含量以及合成甾体皂苷关键基因的表达水平,从生理生态递进到分子层面,对共生机制进行剖析。研究表明,4株滇重楼内真菌都具有固氮和产吲哚乙酸(indoleacetic acid,IAA)的能力,并促进生物量的积累;木霉菌(8-6)能显著提高滇重楼幼苗的光合能力;人参生柱隔孢(8-13)促进了皂苷含量的积累;实时定量PCR显示总皂苷积累可能与皂苷合成途径基因的上调表达有关。本研究结果为内生真菌用作滇重楼促生长的微生物菌肥的研究提供理论依据。Endophytic fungi can p romote plant growth through metabolism and stimulate the accumulation of useful components in medicinal plants.In order to solve problems that the slow growth and the long period required for the accumulation of medicinal components of Pairs polyphylla var.yunnanensis,In this study,four endophytic fungi were selected and inoculated into seedlings of Paris polyphylla var.yunnanensis.The detection of botanical traits,photosynthetic characteristics,steroidal saponin content and expression levels of key genes about saponins expression level of key genes of Paris polyphylla steroidal saponins,and dissecting the mechanisms of symbiosis,moving from physiological ecological to molecular levels.The results showed that four endophytic fun gi had the ability to fix nitrogen and produce indoleacetic acid(IAA),and promote biomass accumulation;Trichoderma viride(8-6) could significantly improve the photosynthetic ability of Paris polyphylla var.yunnanensis;Cylindrocarpon sp.(8-13) promotes the accumulation of saponins;real time PCR showed that the accumulation of total saponins might be related to the up regulation of saponin synthesis pathway genes.These results provide a theoretical basis for the study of endophytic fungi used as microbial fertilizer to promote the growth of Paris polyphylla var.yunnanensis.

关 键 词：滇重楼 内生真菌 促生能力 

分 类 号：S567.239[农业科学—中草药栽培]