合肥地区献血者血液筛查非重复反应性标本与OBI标本间HBV血清学特征分析  

HBV serological characteristics between NRR samples and OBI samples of from blood donors in Hefei

作　　者：李明睿 王婷 陈志超 王婷婷 何晴 LI Mingrui;WANG Ting;CHEN Zhichao;WANG Tingting;HE Qing(Anhui Blood Center,Hefei 230031,China)

机构地区：[1]安徽省血液中心,安徽合肥230031

出　　处：《中国输血杂志》2024年第4期405-411,共7页Chinese Journal of Blood Transfusion

摘　　要：目的 了解血液筛查非重复反应性标本与隐匿性乙型肝炎病毒感染(OBI)标本间HBV血清学特征的相关性。方法 收集本科室2021年1月—2023年1月血液筛查ELISA结果均为阴性,仅NAT反应性的标本共144份,其中TMA法联检反应性标本92份,PCR法单人份检测HBV DNA反应性标本52份。联检反应性标本补充TMA法鉴别检测和PCR法单人份检测,2种方法检测均无反应性的标本纳入NRR标本组,任1种方法检出HBV DNA反应性的标本纳入OBI标本组。对2组标本完成HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc检测,分析NRR标本与OBI标本在血清学模式和阳性率上是否存在差异。结果 联检反应性标本补充检测均阴性标本53份,纳入NRR标本组。91份标本在任1种方法中检测出HBV DNA反应性,纳入OBI标本组。2组标本血清学检测均未检出HBsAg和HBeAg,抗-HBs检出率NRR标本组为64.15%,OBI标本组为47.25%;抗-HBc检出率NRR标本组为86.79%,OBI标本组为94.51%;抗-HBe检出率NRR标本组为35.85%,OBI标本组为52.75%。2组标本血清学模式:NRR标本组表现最多的模式为抗-HBs+、抗-HBc+(32.08%),OBI标本组表现最多的模式为抗-HBe+、抗-HBc+(37.36%)。结论 NRR标本与OBI标本在HBV血清学检测中的部分检测结果间存在差异,但NRR标本中较高的抗-HBc阳性率提示仍有较高的可能存在HBV感染风险。Objective To analyze the correlation of HBV serological characteristics between non-reproducible reactivity(NRR)samples and occult hepatitis B virus infection(OBI)samples for blood screening.Methods A total of 144 samples with negative ELISA(HBV,HCV and HIV test)results and reactive nucleic acid tests(NAT)were collected from January 2021 to January 2023 in Anhui Blood Center,including 92 reactive samples by TMA method(combined ID-NAT)and 52 HBV DNA reactive samples by PCR method(ID-NAT).Supplementary differential testing and ID-NAT by PCR were performed on the reactive samples of the combined ID-NAT,samples that were non-reactive by both differential testing and ID-NAT by PCR were included in the NRR group,and samples that were reactive for HBV DNA detected by either method were included in the OBI group.Supplemented with HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc tests,the differences in serological pattern and positive rate between NRR samples and OBI samples were analyzed.Results A total of 53 samples were negative for differential testing and ID-NAT and were included in the NRR group,91 samples were detected as HBV DNA reactive in either method and were included in the OBI group.HBsAg and HBeAg were not detected by serological testing in either group.The detection rates of anti-HBs,anti-HBc and anti-HBe in the NRR group and the OBI group were 64.15%vs 47.25%,86.79%vs 94.51%,35.85%vs 52.75%,respectively.Comparison of serological patterns between the two groups:the most frequent pattern in the NRR group was anti-HBs(+)and anti-HBc(+)(32.08%),and the most frequent pattern in the OBI group was anti-HBe(+)and anti-HBc(+)(37.36%).Conclusion There were differences in some of the test results between the NRR samples and the OBI samples in HBV serological testing,and higher anti-HBc positive rate in the NRR samples suggests a higher possibility of HBV infection.

关 键 词：乙型肝炎病毒 核酸重复检测非反应性 OBI HBV血清学阳性 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学] R373.2