冷沉淀凝血因子融化后储存温度和时间对凝血因子的影响  

Effect of storage temperature and time on coagulation factor after cryoprecipitated antihemophilic factor melting

作　　者：王媛 刘国英[1] 孔大为[1] 李建斌[1] 金新莉[1] 张玉红[1] 葛文超[1] 成琳[1] 刘佳璇 刘玉振[1] WANG Yuan;LIU Guoying;KONG Dawei;LI Jianbin;JIN Xinli;ZHANG Yuhong;GE Wenchao;CHENG Lin;LIU Jiaxuan;LIU Yuzhen(Henan Red Cross Blood Center,Zhengzhou 450012,China)

机构地区：[1]河南省红十字血液中心,河南郑州450012

出　　处：《中国输血杂志》2024年第4期425-430,共6页Chinese Journal of Blood Transfusion

基　　金：河南省医学科技攻关计划项目(2018020587)。

摘　　要：目的 通过研究比较冷沉淀凝血因子融化后不同的储存温度和时间对凝血因子的影响,为行业标准的制定提供参考依据。方法 2021年6月—2023年5月,每月抽检4袋共96袋冷沉淀凝血因子,当月抽检并及时检测。冷沉淀凝血因子37℃水浴融化后对轻中度脂血予以标记,每袋冷沉淀凝血因子和2个50 mL转移袋用无菌接驳机分装成2袋2组各20 mL,1组放置4℃冰箱,另1组放置22℃水浴箱中,放置时间均为0 h、4 h、8 h、12 h、24 h、48 h,然后再各自时间无菌取样2 mL放入试管中,用加样枪在另1试管中加入样品1 mL和缓冲液3 mL混匀上机进行检测。随机选取60袋无轻中度脂血冷沉淀凝血因子的实验数据,采用SPSS21.0进行统计分析。结果 融化后的冷沉淀凝血因子贮放0 h、4 h、8 h、12 h、24 h、48 h检测2组凝血因子平均含量及增长率:(1)储存4℃,Ⅷ因子含量依次为118.62、111.57(-5.95%)、105.51(-11.05%)、103.30(-12.92%)、94.35(-20.46%)、83.25(-29.82%)IU/袋;储存22℃,Ⅷ因子含量依次为118.62、112.69(-5.00%)、111.41(-6.08%)、109.01(-8.10%)、101.55(-14.39%)、92.75(-21.81%)IU/袋,2组储存结果比较,在4℃24 h和22℃48 h,Ⅷ因子含量均有显著统计学意义(P<0.01),且在22℃储存,Ⅷ因子衰减速度更慢;(2)储存4℃,Ⅴ因子含量依次为41.19、41.31(0.29%)、40.52(-1.64%)、40.27(-2.23%)、39.05(-5.19%)、36.99(-10.21%)IU/袋;储存22℃,Ⅴ因子含量依次为41.19、41.71(1.25%)、42.54(3.28%)、41.94(1.80%)、39.21(-4.80%)、35.64(-13.48%)IU/袋,2组储存结果比较,在4℃48 h和22℃48 hⅤ因子含量分别有统计学意义(P<0.05)和有显著统计学意义(P<0.01),且在22℃储存,Ⅴ因子衰减速度更快;(3)储存4℃,Fbg含量依次为268.86、268.17(-0.26%)、262.46(-2.38%)、270.50(0.61%)、267.52(-0.50%)、261.92(-2.58%)mg/袋;储存22℃,Fbg含量依次为268.86、265.86(-1.12%)、264.12(-1.77%)、265.89(-1.11%)、266.04(-1.05%)、261.04(-2.91%)mg/袋,2组各时间段与0 h含量比�Objective To study and compare the effects of different storage temperature and time on coagulation factor after cryoprecipitated antihemophilic factor(CAF)melting,and to provide reference for the establishment of industry standards.Methods From June 2021 to May 2023,a total of 96 bags of CAF were sampled in 4 bags per month,and timely detected in the same month.After the CAF was melted in a 37℃water bath,the mild to moderate lipemic blood was labeled.Each bag of CAF and two 50 mL transfer bags were divided into two bags and two groups of 20 mL each using a sterile adapter.One group was placed in a 4℃refrigerator and the other in a 22℃water bath for 0 h,4 h,8 h,12 h,24 h and 48 h.Then 2 mL of aseptic sample was taken separately and put into the test tube,and 1mL of sample and 3 mL of buffer were added into the other test tube with the sampling gun and mixed on the machine for testing.The experimental data of 60 bags without mild to moderate lipemic blood cryoprecipitation and coagulation factor were randomly selected and statistically analyzed by SPSS21.0.Results After melting,CAF was stored for 0 h,4 h,8 h,12 h,24 h and 48 h to detect the average content and growth rate of coagulation factor in the two groups:1)Storage at 4℃,factorⅧcontent was 118.62,111.57(-5.95%),105.51(-11.05%),103.30(-12.92%),94.35(-20.46%)and 83.25(-29.82%)IU/bag,respectively;Storage at 22℃,the factorⅧcontent was 118.62,112.69(-5.00%),111.41(-6.08%),109.01(-8.10%),101.55(-14.39%)and 92.75(-21.81%)IU/bag,and the storage results of the two groups were compared.At 24 h at 4℃and 48 h at 22℃,the content of factorⅧhad significant statistical significance(P<0.01),and when stored at 22℃,the decay rate of factorⅧwas slower;2)When stored at 4℃,the content of factor V was 41.19,41.31(0.29%),40.52(-1.64%),40.27(-2.23%),39.05(-5.19%)and 36.99(-10.21%)IU/bag,respectively;Stored at 22℃,the factor V content was 41.19,41.71(1.25%),42.54(3.28%),41.94(1.80%),39.21(-4.80%)and 35.64(-13.48%)IU/bag,respectively.Comparison of storage r

关 键 词：冷沉淀凝血因子 CAF Ⅷ因子 Ⅴ因子 FBG 纤维蛋白原 储存温度 储存时间 

分 类 号：R446.11[医药卫生—诊断学] R457.12[医药卫生—临床医学]