海南地区某门诊泌尿生殖道沙眼衣原体的感染及基因分型  

Urogenital Chlamydia trachomatis infection and genotyping in an outpatient clinic in Hainan

作　　者：吴捷 林丽华 王碧 王皇玉 钟娜 WU Jie;LIN Lihua;WANG Bi;WANG Huangyu;ZHONG Na(The Fifth People's Hospital of Hainan Province,Haikou,Hainan 570100,China)

机构地区：[1]海南省第五人民医院,海南海口570100

出　　处：《中国热带医学》2024年第3期315-319,共5页China Tropical Medicine

基　　金：海南省卫生健康委员会立项课题(No.21A200304)。

摘　　要：目的对海南省性病门诊泌尿生殖道沙眼衣原体(Chlamydia trachomatis,CT)感染状况及其基因分型进行初步研究,了解泌尿生殖道CT感染人群的流行特点和型别特征,为制定科学防护策略和措施提供依据。方法收集2018—2022年就诊于海南省第五人民医院性病门诊的5551份男性尿道拭子标本和女性宫颈拭子标本,利用实时荧光定量聚合酶链反应(real time fluorescent quantitative polymerase chain reaction,RT-PCR)法检测CT感染情况,取部分CT阳性者的DNA进行巢式PCR扩增OMPL基因,并对阳性结果进行测序,测序结果上传至BLAST网站查找序列的相似性,构建系统发育树,确定基因型。结果5551例感染者有846例CT感染阳性,阳性感染率为15.2%。其中男性CTDNA阳性检出率为18.6%,女性为13.4%,男性阳性检出率高于女性;各年龄段CT-DNA的阳性检出率差异有统计学意义(P<0.05),20~<30岁的CT-DNA阳性率最高为58.0%,50岁及以上CT-DNA的阳性率最低为1.0%;不同季节间CTDNA的阳性检出率差异有统计学意义(P<0.05),夏季最高为36.4%,冬季最低为9.6%。取海南地区部分标本进行CTOMPL基因VS1~VS2核苷酸序列分型,共测得6个基因型,包括D、E、G、F、J和K型,其中F型为主要流行优势型。结论海南地区CT感染与性别、年龄和季节有关,基因感染型呈现多元化,F型为主要流行优势型。在今后的性病防治工作中有必要进一步加强育龄期男女生殖道CT感染的筛查力度,提高生育率。Objective To conduct preliminary research on the prevalence and genotyping of urogenital Chlamydia trachomatis(CT)infection in sexually transmitted disease(STD)clinics in Hainan Province,to understand the epidemiological characteristics and genotype features of the population infected with urogenital CT,and to provide evidence for the formulation of scientific prevention strategies and measures.Methods From 2018 to 2022,a total of 5551 male urethral swabs and female cervical swabs were collected for detection of CT infection by real-time fluorescent quantitative polymerase chain reaction(RT-PCR).The OMPL gene was amplified from the DNA of some CT positive individuals by nested PCR,and the positive results were sequenced.Sequencing results were uploaded to BLAST website to find sequence similarity and construct a phylogenetic tree to determine the genotype.Results Out of the 5551 tested patients,846 were positive for Chlamydia trachomatis infection,with a positivity rate of 15.2%,the positive detection rate of CT-DNA was 18.6%in male and 13.4%in female,the positive detection rate of male was higher than that of female.There were statistically significant differences in the CT-DNA positivite detection rate among different age groups(P<0.05),and the highest positivite rate CT-DNA was 58.0%in 20-<30 years old,while it was the lowest,at 1.0%,in those over 50;there were also significant differences in CT-DNA positivity detection rate between seasons(P<0.05),with the highest being 36.4%in the summer and the lowest at 9.6%in the winter.Genotyping of the CT-OMPL gene VS1-VS2 nucleotide sequence in some samples from the Hainan region identified six genotypes,including types D,E,G,F,J,and K,with type F being the main prevalent dominant type.Conclusions CT infection in Hainan is associated with gender,age,and season,and the genotypes are diversified.It is necessary to further strengthen the screening of CT infection in the reproductive tract of men and women of childbearing age in the future STD prevention and treatment work

关 键 词：泌尿生殖道 沙眼衣原体 OMPL基因 分型研究 

分 类 号：R3741[医药卫生—病原生物学]