肠道微生态对子痫前期发病的影响  

Impact of gut microbiota on the onset of pre-eclampsia

作　　者：王晓艳[1] 李莲英[1] 王燕侠[1] 张翰儒[1] 刘婷婷[1] WANG Xiao-yan;LI Lian-ying;WANG Yan-xia;ZHANG Han-ru;LIU Ting-ting(Department of Gynaecology and Obstetrics,Gansu Maternal and Child Health Hospital,Lanzhou 730050,Gansu,China)

机构地区：[1]甘肃省妇幼保健院妇产科,甘肃兰州730050

出　　处：《广东医学》2024年第2期133-138,共6页Guangdong Medical Journal

基　　金：甘肃省自然科学基金项目(21JR7RA663)。

摘　　要：目的探讨肠道微生态对子痫前期(pre-eclampsia,PE)发病的影响及机制。方法选取2021年1月至2022年6月甘肃省妇幼保健院妇产科收治的40例PE患者及40例健康孕妇作为研究对象,按照孕期不同分为4组,26周PE孕妇作为A1组(n=20),36周PE孕妇作为A2组(n=20);26周健康孕妇作为B1组(n=20),36周健康孕妇作为B2组(n=20)。收集4组研究对象清晨粪便标本,提取粪便细菌DNA基因,采用16S rDNA的扩增及测序,得到肠道菌群α多样性及肠道菌群差异性。结果B2组Chao1及Observed-Species指数均低于B1组(P<0.001);与B1组相比,B2组艰难梭状芽胞杆菌(Clostridium)、马氏羊蹄菌(Fournierella)、普雷沃菌(Prevotella)、梭杆菌属(Fusobacterium)丰度升高,双歧杆菌(Bifidobacterium)及普拉梭菌(Faecalibacterium)的丰度降低。A1组Chao1及Observed-Species指数均低于B1组(P<0.001)。与B1组相比,A1组Clostridium、Fournierella、Prevotella、Fusobacterium丰度升高,Bifidobacterium及Faecalibacterium的丰度降低。A2组Chao1及Observed-Species指数均第于B2组(P<0.001);与B2组相比,A2组Clostridium、Fournierella、Prevotella、Fusobacterium丰度升高,Bifidobacterium及Faecalibacterium的丰度降低。A2组Chao1及Observed-Species指数均低于A1组(P<0.001);与A1组相比,A2组Clostridium、Fournierella、Prevotella、Fusobacterium丰度升高,Bifidobacterium及Faecalibacterium的丰度降低。结论健康孕妇会随着孕周的延长肠道菌群α多样性降低,致病菌丰度增加;PE肠道菌群致病性高于健康孕妇,且α多样性降低;PE会随着孕周的增加,肠道菌群α多样性降低,且上述致病菌丰度升高,有益菌降低,认为加强PE机体肠道菌群的监测对于预防炎症性疾病具有重要意义。Objective To elucidate the influence of gut microbiota on the pathogenesis of pre-eclampsia(PE)and investigate the underlying mechanisms.Methods A cohort of 40 PE patients and 40 healthy pregnant women,ad-mitted to the Department of Obstetrics and Gynecology at Gansu Maternal and Child Health Hospital from January 2021 to June 2022,constituted the study population.Participants were categorized into four groups based on different gestational periods:Group A1(PE patients at 26 weeks,n=20),Group A2(PE patients at 36 weeks,n=20),Group BI(healthy pregnant women at 26 weeks,n=20),and Group B2(healthy pregnant women at 36 weeks,n=20).Morning fecal samples were collected from all groups,and bacterial DNA was extracted for 16S rDNA amplification and sequencing to as-sess gut microbiota alpha diversity and diferential composition.Results Chaol and Observed-Species indices in Group B2 were significantly lower than those in Group B1(P<0.001).Compared to Group B1,Group B2 exhibited increased abundance of Clostridium,Fournierella,Prevotella,and Fusobacterium,along with decreased abundance of Bifidobacteri-um and Faecalibacterium.Group A1 demonstrated lower Chaol and Observed-Species indices than Group B1(P<0.001),displaying analogous microbial composition changes as observed in Group B2.Similar patterns were noted in Group A2 compared to Group B2.Furthermore,Group A2 displayed lower Chaol and Observed-Species indices than Group A1(P<0.001),with comparable shifts in microbial composition.Conclusion In healthy pregnant women,a re-duction in gut microbiota alpha diversity occurs with advancing gestational weeks,resulting in an increase in pathogenic bacteria.The gut microbiota of PE patients exhibits higher pathogenicity than that of healthy pregnant women,character-ized by reduced alpha diversity.Additionally,as gestational weeks increase in PE,gut microbiota alpha diversity decrea-ses,pathogenic bacteria increase,and beneficial bacteria decrease.Vigilant monitoring of the gut microbiota in PE individuals holds paramount importa

关 键 词：子痫前期 肠道菌群 Α多样性 致病菌 有益菌 

分 类 号：R714.2[医药卫生—妇产科学] R378.3[医药卫生—临床医学]