基于HIV-1的pol基因区序列分析我国CRF07_BC毒株的跨地区传播特征  

Characterization of cross-regional transmission on the predominantly endemic CRF07_BC strain in China through the sequence of the pol gene region of HIV-1

作　　者：王栋 冯毅[1] 郝静静 胡红萍 李方园 李佳璐 廖玲洁[1] 阮玉华[1] 邵一鸣 邢辉[1] WANG Dong;FENG Yi;HAO Jingjing;HU Hongping;LI Fangyuan;LI Jialu;LIAO Lingjie;RUAN Yuhua;SHAO Yiming;XING Hui(National Center for AIDS/STD Control and Prevention,China Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京102206

出　　处：《中国艾滋病性病》2024年第3期232-239,共8页Chinese Journal of Aids & STD

基　　金：“十四五”国家重点研发计划(2022YFC2305201)。

摘　　要：目的通过构建我国HIV-1的CRF07_BC毒株pol基因区序列的分子网络,分析CRF07_BC毒株在我国的跨地区传播特征,为促进我国HIV精准防控提供数据支持。方法使用FastTree与中国艾滋病病毒基因序列数据平台(https://nmdc.cn/hiv/)的亚型判别工具进行亚型判别,筛选出我国CRF07_BC毒株的pol基因序列构建分子网络,使用单因素与多因素Logistic回归分析入网率与跨地区连接影响因素,计算跨地区聚类系数等分子网络分析指标描述不同省份之间的传播连接和强度特征。结果共收集到我国HIV-1毒株pol基因区序列81612条,其中CRF07_BC毒株26181条序列,在0.5%基因距离阈值下构建分子网络,形成2028个分子网络,最大的分子网络含有6262个节点,命名为07-1-1,节点来自全国31个省(自治区、直辖市),以男男性行为传播途径为主(32.0%,2005/6262)。跨地区分析指标显示07-1-1分子网络中68.9%为跨地区连接边,80.6%为跨地区节点;跨地区聚类系数显示分子网络07-1-1的跨地区传播以广东省(66.6%,79114/118819)与北京市(27.9%,33159/118819)为中心,发生在我国华东地区、西南地区、华南地区和华中地区等地区间。结论我国HIV跨地区传播中CRF07_BC毒株起很强的主导作用,广东省与北京市处于跨地区传播的中心位置,有效控制HIV毒株在我国的跨地区传播,将成为我国艾滋病防控高质量发展的重要举措之一。Objective In order to provide data support for the promotion of precise HIV prevention and control,this article analyze the cross-regional transmission characteristics of the CRF07_BC strain in China,by constructing a molecular network of the sequence of the pol gene region of the CRF07_BC strain of HIV-1 in China.Methods FastTree and the subtyping tool of the China HIV Genetic Sequence Data Platform(https://nmdc.cn/hiv/)were used to discriminate subtypes,and the pol gene sequences of the CRF07_BC strain in China were screened to construct a molecular network.Univariate and multivariate logistic regression analysis were used to identify the factors influencing access rate and crossregional connectivity.Multi-jurisdictional analysis indexes,such as cross-region clustering coefficients,were calculated to characterize the transmission connectivity and intensity among different provinces.Results A total of 81612 sequences of the pol gene region of HIV-1 strains in China were collected,including 26181 sequences of CRF07_BC strain.Under the 0.5%gene distance threshold,2028 molecular networks were constructed.The largest molecular network,named 07-1-1,containing 6262 nodes from 31 provinces(autonomous regions and municipalities)nationwide,and the predominant transmission route was through male-to-male sex behavior(32.0%,2005/6262).The indicator of multi-jurisdictional transmission analysis showed that 68.9%of the molecular networks in 07-1-1 were multi-jurisdictional connecting edges,and 80.6%were multi-jurisdictional transmission nodes.The multi-jurisdictional clustering coefficients showed that the multi-jurisdictional transmission of molecular network 07-1-1 occurs mainly in Guangdong Province(66.6%,79114/118819)and Beijing Municipality(27.9%,33159/118819),as well as in other regions of China,including the east,southwest,south,and central regions.Conclusions The CRF07_BC strain plays a dominant role in the multi-jurisdictional transmission of HIV in China,with Guangdong Province and Beijing Municipality at the center

关 键 词：1型艾滋病病毒 跨地区传播 分子网络 聚类系数 

分 类 号：R373.9[医药卫生—病原生物学] R512.91[医药卫生—基础医学]