Slc43a2^(+)T cell metastasis from spleen to brain in RGNNV infected teleost  

作　　者：Qing Wang Yali Liu Minlin Zhang Min Yang Jiantao Liang Xiaoling Zuo Shaowen Wang Xianze Jia Huihong Zhao Han Jiang Qiang Lin Qiwei Qin 

机构地区：[1]College of Marine Sciences,South China Agricultural University,Guangzhou 510642,China [2]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [3]Nansha-South China Agricultural University Fishery Research Institute,Guangzhou 511457,China [4]Joint University Laboratory of Guangdong Province,Hong Kong and Marco Region on Marine Bioresource Conservation and Exploitation,Guangzhou 510642,China [5]University of Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Science China(Life Sciences)》2024年第4期733-744,共12页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFD2400502);the National Natural Science Foundation of China(42176103,41825013,42230409,42276127);the Guangdong Provincial Natural Science Foundation(2022A1515012505);the Key-Area Research and Development Program of Guangdong Province(2021B0202040002);China Agricultural Research System(CARS-47-G16)。

摘　　要：The origin of T cells in the teleost's brain is unclear.While viewing the central nervous system(CNS)as immune privileged has been widely accepted,previous studies suggest that T cells residing in the thymus but not in the spleen of the teleost play an essential role in communicating with the peripheral organs.Here,we identified nine T cell subpopulations in the thymus and spleen of orange-spotted grouper(Epinephelus coioices)through single-cell RNA-sequencing analysis.After viral CNS infection with red-spotted grouper nervous necrosis virus(RGNNV),the number of slc43a2^(+)T cells synchronously increased in the spleen and brain.During the infection tests in asplenic zebrafish(tlx1^▲zebrafish model),no increase in the number of slc43a2^(+)T cells was observed in the brain.Single-cell transcriptomic analysis indicated that slc43a2^(+)T cells mature and functionally differentiate within the spleen and then migrate into the brain to trigger an immune response.This study suggests a novel route for T cell migration from the spleen to the brain during viral infection in fish.

关 键 词：central nervous system slc43a2^(+)T cell RGNNV T cell migration TELEOST 

分 类 号：S943[农业科学—水产养殖]