Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis  

作　　者：NIE Cunpeng MA Tianran CHEN Tingting CHU Xia 

机构地区：[1]State Key Laboratory of Chemo/Bio-Sensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha,410082,P.R.China

出　　处：《Chemical Research in Chinese Universities》2024年第2期333-342,共10页高等学校化学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21991080,22374042);the Science and Technology Major Project of Hunan Province,China(No.2021SK1020).

摘　　要：In molecular engineering,designing and synthesizing molecular machines with capable of performing complex tasks,remains a formidable challenge.DNA is an excellent candidate for building molecular robots because it is highly programmable.Here,we present an artificial nanorobot,in which a DNA cube serves as the inert‘body’,and nucleic acid catalysts based on an enzymatic nicking reaction act as the‘legs’for walking.The nanorobot can execute a series of actions,such as‘start’,‘turn’,and‘stop’when it walks along a predefined track.Its performance could be confirmed and monitored by using an atomic force microscope(AFM)and fluorescence spectroscopy.Inspired by biological machines,we artificially designed a series of specialized tasks that combined walking with control of cargo transport and catalysis.Real-time fluorescence kinetics curves provide monitoring signals for cargo transport and catalytic processes.Our work can enrich the toolbox of DNA machinery and has great potential for engineering molecular nanofactories.

关 键 词：DNA origami DNA nanorobot Controlled cargo transport DNAzyme catalysis 

分 类 号：O64[理学—物理化学]