槲皮素抑制CD38/ERK通路介导的肺泡上皮细胞炎性反应  

Quercetin inhibits CD38/ERK pathway-mediated inflammatory response of alveolar epithelial cell

作　　者：俞丽君 张辉青 方卉 杜宇娜 游静[1,2] 李蓉 YU Lijun;ZHANG Huiqing;FANG Hui;DU Yuna;YOU Jing;LI Rong(Jiangxi College of Medicine,Nanchang University;The First Affiliated Hospital of Nanchang Medical College,Jiangxi Provincial People's Hospital,Nanchang 330006,China)

机构地区：[1]南昌大学江西医学院,江西南昌330006 [2]江西省人民医院南昌医学院第一附属医院,江西南昌330006

出　　处：《实验与检验医学》2024年第1期1-5,16,共6页Experimental and Laboratory Medicine

基　　金：江西省教育厅科学技术研究项目,编号GJJ200211;国家自然科学基金项目,编号NSFC31960165。

摘　　要：目的 探讨肺部感染患者外周血中白细胞CD38表达和炎性因子表达的潜在联系,以及槲皮素对LPS诱导肺泡上皮细胞炎性反应的抑制机制。方法 收集2023年5月至2023年12月在江西省人民医院收治的肺部感染患者血液标本作为感染组(n=50),另选取本院同期健康体检者血液标本作为对照组(n=50),采用流式细胞术检测外周血中白细胞CD38和炎性因子水平表达。生物信息学预测槲皮素和CD38的关系,CCK-8法检测不同浓度的LPS和不同浓度的槲皮素对MLE-12细胞活力的影响,免疫印迹法检测CD38、IL-18、TNF-α表达水平和MAPK通路相关蛋白的表达。结果 肺部感染组外周血白细胞和淋巴细胞表面CD38表达水平均显著高于对照组(P<0.05),肺部感染组IL-6(P<0.01)和IL-10(P<0.05)水平也显著高于对照组;细胞实验结果表明,与LPS组相比,LPS+槲皮素组的CD38、IL-18、TNF-α和p-ERK/ERK水平显著下降(P<0.05)。结论 肺部感染外周血白细胞中CD38表达上调,主要以淋巴细胞CD38表达升高为主,CD38促进外周血中炎性因子的释放是肺部炎症的发生机制之一。CD38是槲皮素调控的炎性反应的潜在靶点,槲皮素可以通过抑制CD38/ERK通路减少MLE-12细胞中由LPS诱导的炎性因子IL-18和TNF-α的释放。Objective To investigate the potential relationship between the expression of CD38 and inflammatory factors in peripheral blood leukocytes of patients with pulmonary infection,and the inhibitory mechanism of quercetin on LPS-induced inflammatory response of alveolar epithelial cells.Methods Blood samples of patients with pulmonary infection admitted to Jiangxi Provincial People's Hospital from May 2023 to December 2023 were collected as the infection group(n=50),and blood samples of healthy subjects in our hospital during the same period were selected as the control group(n=50).Flow cytometry was used to detect the levels of CD38 and inflammatory factors in peripheral blood cells.Bioinformatics predicted the relationship between quercetin and CD38,CCK-8 method was used to detect the effects of LPS and quercetin on the viability of MLE-12 cells,and western blot was used to detect the expression levels of CD38,IL-18,TNF-αand MAPK pathway-related proteins.Results The expression levels of CD38 on peripheral white blood cells and lymphocytes in pulmonary infection group were significantly higher than those in control group(P<0.05),and the levels of IL-6(P<0.01)and IL-10(P<0.05)in pulmonary infection group were also significantly higher than those in control group.The results of cell experiment showed that CD38,IL-18,TNF-αand P-ERK/ERK levels in LPS+quercetin group were significantly decreased compared with LPS group(P<0.05).Conclusion CD38 expression is up-regulated in peripheral blood leukocytes of pulmonary infection,mainly in lymphocytes.CD38 promotes the release of inflammatory factors in peripheral blood,which is one of the mechanisms of pulmonary inflammation.CD38 is a potential target of quercetin regulated inflammatory responses,and quercetin can reduce the release of LPS-induced inflammatory factors IL-18 and TNF-αin MLE-12 cells by inhibiting the CD38/ERK pathway.

关 键 词：分化抗原簇38 肺部感染 槲皮素 炎性反应 细胞外调节蛋白激酶 

分 类 号：R563[医药卫生—呼吸系统]