悬铃木组织培养再生体系的建立  

Establishment of Tissue Culture Regeneration System of Sycamore

作　　者：孟汇然 郭尚敬[1] 张演义[1] 隋超 王德颖 吕陆佳 MENG Huiran;GUO Shangjing;ZHANG Yanyi;SUI Chao;WANG Deying;LYU Lujia(Agricultural Science and Engineering School,Liaocheng University,Liaocheng Shandong 252000)

机构地区：[1]聊城大学农学与农业工程学院,山东聊城252000

出　　处：《现代农业科技》2024年第8期78-80,共3页Modern Agricultural Science and Technology

基　　金：山东省农业科技园区产业提升工程项目(2019YQ035)。

摘　　要：为了优化建立悬铃木组织培养再生体系,本研究以健壮、无病虫害的一年生茎段作为外植体,考察不同的灭菌方法和激素配比对外植体灭菌、启动、增殖、生根的影响。结果表明:悬铃木茎段的最佳灭菌方法是75%乙醇灭菌30 s+0.1%HgCl2消毒17 min,污染率可降至20%;最适宜的启动培养基为MS+ZT 4 mg/L+IAA 1.0 mg/L,启动率可达到65%;最适宜的增殖培养基为MS+ZT 3 mg/L+IAA 1.0 mg/L,增殖系数可达到1.95;最适宜的生根培养基为1/2 MS+IBA 0.1 mg/L,生根率可达到75%。In order to optimize and establish the tissue culture regeneration system of sycamore,this study took healthy and pest free annual stem segments as the explants,and investigated the effects of different disinfection methods and hormone ratios on disinfection,initiation,proliferation and rooting of explants.The results showed that the optimal disinfection method for the stem explants of sycamore was 75%ethanol disinfection for 30 s+0.1%HgCl2 disinfection for 17 min,and the contamination rate could reduce to 20%.The most suitable medium for initiation culture was MS+ZT 4 mg/L+IAA 1.0 mg/L,and the initiation rate could reach 65%.The most suitable proliferation medium was MS+ZT 3 mg/L+IAA 1.0 mg/L,and the proliferation coefficient could reach 1.95.The most suitable rooting medium was 1/2 MS+IBA 0.1 mg/L,and the rooting rate could reach 75%.

关 键 词：悬铃木 组织培养 外植体 再生体系 

分 类 号：S792.99[农业科学—林木遗传育种]