妊娠期糖尿病孕妇个体化饮食干预前后肠道菌群的对比  

Comparison of intestinal flora in pregnant women with gestational diabetes mellitus before and after individualized diet intervention

作　　者：赵芸芸 李莉[2] 马依拉·买买提[3] Zhao Yunyun;Li Li;Mayila·Maimaiti(School of Public Health,Xinjiang Medical University,State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Urumqi 830011,China;Department of Clinical Nutrition,the First Affiliated Hospital of Xinjiang Medical University,State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Urumqi 830054,China;Department of Clinical Nutrition,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]省部共建中亚高发病成因与防治国家重点实验室,新疆医科大学公共卫生学院,乌鲁木齐830011 [2]省部共建中亚高发病成因与防治国家重点实验室,新疆医科大学第一附属医院临床营养科,乌鲁木齐830054 [3]新疆医科大学第一附属医院临床营养科,乌鲁木齐830054

出　　处：《中华临床营养杂志》2024年第1期15-24,共10页Chinese Journal of Clinical Nutrition

基　　金：省部共建中亚高发病成因与防治国家重点实验室开放课题资助项目(SKL-HIDCA-2021-DX7)。

摘　　要：目的利用16S rDNA全长高通量测序技术,探索妊娠期糖尿病(gestational diabetes mellitus,GDM)孕妇饮食干预前后肠道菌群的变化及与口服葡萄糖耐量试验(oral glucose tolerance test,OGTT)结果的相关性。方法选取2022年7月至2023年3月在新疆医科大学第一附属医院定期产检的孕妇30例,根据24~28周OGTT结果,分为研究组(15例)和正常组(15例)。收集其一般资料和实验室相关检查指标,同时对研究组孕妇进行个体化饮食干预,对正常组孕妇给予常规饮食指导干预,干预时间均为2周,分别收集两组干预前、后的粪便,共收集到60份粪便样本。通过PacBio测序平台,利用单分子实时测序对受试者粪便DNA进行第三代微生物16S rDNA全长多样性测序,比较两组干预前、后肠道菌群的结构、丰度及多样性。结果两组孕妇的菌群丰度有显著差异的物种共有74种,其中54种在研究组中富集。纳入OGTT结果作为环境因子进行相关性分析显示,毛螺菌(P=0.002)和未分类的细菌(P=0.035)与OGTT 0 h血糖呈正相关,差异有统计学意义(P<0.05);厚壁菌(P=0.018)、颤螺菌(P=0.045)与OGTT 0 h血糖呈负相关,差异有统计学意义(P<0.05);毛螺菌(P=0.027)、未分类的细菌(P=0.028)与OGTT 1 h血糖呈正相关,差异有统计学意义(P<0.05),颤螺菌与OGTT 1 h血糖呈负相关(P=0.025),差异有统计学意义(P<0.05);毛螺菌(P=0.027)与OGTT 2 h血糖呈正相关,差异有统计学意义(P<0.05)。结论GDM孕妇经过个体化饮食干预后,肠道菌群中的优势菌发生了改变,GDM孕妇空腹血糖较干预前降低,由此可推断GDM孕妇可通过个体化饮食干预调节紊乱的肠道菌群,这一发现为饮食干预治疗妊娠期糖尿病提供有力的支持。Objective To study the changes of intestinal flora before and after dietary intervention in pregnant women with gestational diabetes mellitus(GDM)and explore its correlation with the results of oral glucose tolerance test(OGTT),using third generation high-throughput sequencing of full-length 16S rDNA.Methods Thirty pregnant women who received regular antenatal care in the First Affiliated Hospital of Xinjiang Medical University between July 2022 and March 2023 were selected.Demographic data and laboratory examination results of these pregnant women were collected.The pregnant women with GDM(the GDM group)were given individualized dietary intervention,while the control group were given routine dietary guidance,for a total of 2 weeks.The stool was collected before and after the intervention in both groups,and a total of 60 stool samples were collected.Through the PacBio Sequencing platform,the third generation sequencing of full-length 16S rDNA was utilized to investigate the intestinal microbiome diversity.The composition,abundance and diversity of intestinal flora were compared between groups,both before and after the intervention.Results There were 74 species showing significant differences in abundance between the two groups of pregnant women,54 of which were enriched in the GDM group.The correlation analysis of blood glucose levels tested by OGTT as an environmental factor showed that Lachnospirales(P=0.002)and unclassified Bacteria(P=0.035)were positively correlated with OGTT-0h blood glucose(P<0.05),while Christensenellales(P=0.018)and Oscillospirales(P=0.045)negatively.Lachnospirales(P=0.027)and unclassified Bacteria(P=0.028)were positively correlated with OGTT-1 h blood glucose(P<0.05),while Oscillospirales(P=0.025)negatively.There was a positive correlation between Lachnospirales(P=0.027)and OGTT-2h blood glucose(P<0.05).Conclusions After the individualized diet intervention,the dominant bacteria of the intestinal flora changed and the fasting blood glucose of declined in pregnant women with GDM.It's a re

关 键 词：肠道菌群 16S rDNA 三代微生物全长高通量测序 妊娠期糖尿病 饮食干预 

分 类 号：R714.256[医药卫生—妇产科学]