磁性表面分子印迹聚合物的制备及对梓醇的选择性吸附  

Preparation of magnetic surface molecular imprinted polymer and its selective adsorption for catalpol

作　　者：秦兵 张伟东 李淑贤[1] QIN Bing;ZHANG Weidong;LI Shuxian(College of Pharmacy,Jiamusi University,Jiamusi 154000,China;College of Pharmacy,Jilin University,Changchun 130000,China)

机构地区：[1]佳木斯大学药学院,佳木斯154000 [2]吉林大学药学院,长春130000

出　　处：《分析试验室》2024年第3期394-401,共8页Chinese Journal of Analysis Laboratory

基　　金：北药与功能食品省“双一流”特色学科项目(2022-TSXK-02)资助。

摘　　要：以Fe_(3)O_(4)@SiO_(2)磁性复合材料为载体,梓醇为模板分子,采用沉淀聚合法合成了新型磁性表面分子印迹聚合物。优化了吸附条件,通过静态与动态吸附实验,考察了新型复合材料的吸附性能。结果表明,该材料对梓醇的最大吸附量为69.07μg/mg,印迹因子为1.97,对梓醇有优良的特异性识别能力。重复使用6次后,回收率仍在85%以上,重复利用性良好。基于此,结合磁固相萃取法,建立了应用于地黄中梓醇富集和检测的高效液相色谱法,梓醇回收率为89.5%~96.8%,相对标准偏差(RSD)≤2.0%,线性范围为0.1~1.15 mg/mL,线性相关系数R^(2)>0.9998,检出限(LOD)为5.4 ng/mL。该新型磁性复合材料为天然产物中活性成分的富集和分析提供了一种新的预处理策略。A novel magnetic surface molecularly imprinted polymer was synthesized by precipitation polymerization using Fe_(3)O_(4) and SiO_(2) magnetic composites as carriers and catalpol as template molecules.The adsorption conditions were optimized and the adsorption performance of the new composite material was investigated by static and dynamic adsorption experiments.The results showed that the maximum adsorption capacity of the material for catalpol was 69.07μg/mg with the imprinted factor(IF)of 1.97,showing excellent specific recognition ability for catalpol.After 6 times of reuse,the recovery was still above 85%,showing good reuse performance.Combined with magnetic solid phase extraction,the high performance liquid chromatography method was established and successfully applied to enrich and determine catalpol in rehmannia glutinosa.The recoveries of catalpol were 89.5%-96.8%,with the relative standard deviations(RSDs)lower than 2%.The linear range was 0.1-1.15 mg/mL,with the correlation coefficient(R^(2))above 0.9998,and the limit of detection(LOD)of 5.4 ng/mL.The new composite magnetic material can provide a promising pretreatment strategy for the enrichment and analysis of active components in natural products.

关 键 词：磁性表面分子印迹聚合物 梓醇 HPLC 磁性材料 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]