云南省西部地区宿主和媒介感染伯氏疏螺旋体的基因多样性调查与分析  

Investigation and analysis of the genetic diversity of Borrelia burgdorferi in host animals and vector ticks from western Yunnan Province

作　　者：何志海 和仙 孙毅 蒋宝贵 邵宗体[1] 张云[1] 李玉琼[1] 浦恩念 姚明国 王帆 别双双 高子厚[1] 江佳富 杜春红[1] HE Zhi-hai;HE Xian;SUN Yi;JIANG Bao-gui;SHAO Zong-ti;ZHANG Yun;LI Yu-qiong;PU En-nian;YAO Ming-guo;WANG Fan;BIE Shuang-shuang;GAO Zi-hou;JIANG Jia-fu;DU Chun-hong(Yunnan Institute for Endemic Diseases Control and Prevention,Yunnan Key Laboratory for Zoonosis Control and Prevention,Dali 671000,China;Guangming Center for Disease Control and Prevention of Shenzhen,Shenzhen 518106,China;Diqing Center for Disease Control and Prevention of Yunnan,Shangri-la 674400,China;State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China)

机构地区：[1]云南省地方病防治所,云南省自然疫源性疾病防控重点实验室,大理671000 [2]深圳市光明区疾病预防控制中心,深圳518106 [3]云南省迪庆州疾病预防控制中心,香格里拉674400 [4]中国人民解放军军事科学院军事医学研究院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《中国人兽共患病学报》2024年第3期208-213,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.U2002219,No.81760607)。

摘　　要：目的了解云南省西部地区宿主动物和媒介蜱感染伯氏疏螺旋体的基因多样性,为进一步掌握该地区莱姆病菌株的特性奠定基础。方法对采用伯氏疏螺旋体5S~23S rRNA间隔区基因筛查阳性的云南西部地区采集的家畜、小型兽类、蜱虫样本,进行伯氏疏螺旋体鞭毛蛋白基因(FLA基因)和16S rRNA基因的检测,综合分析病原体的基因信息,了解当地流行株的生物学特性及流行特征。结果共检测301份样本,共检出7种基因型伯氏疏螺旋体,其中宿主动物共检出5种:B.afzelii(阿弗西尼疏螺旋体)、B.garinii(伽氏疏螺旋体)、B.burgdorferi s.s.(狭义伯氏疏螺旋体)、B.japonica(日本疏螺旋体)、B.valaisiana(法雷斯疏螺旋体);媒介蜱检出7种:B.afzelii(阿弗西尼疏螺旋体)、B.garinii(伽氏疏螺旋体)、B.burgdorferi s.s.(狭义伯氏疏螺旋体)、B.japonica(日本疏螺旋体)、B.valaisiana(法雷斯疏螺旋体)、B.sinica(中华基因型伯氏疏螺旋体)及未定种Borrelia sp.。B.afzelii、B.garinii和B.burgdorferi s.s.为优势流行株,占总阳性的85.04%(256/301)。不同地区、不同来源的相同基因型株基因序列存在种内差异,同一地区的宿主和媒介中检出的相同基因型螺旋体具有较高的同源性和较近的遗传进化关系。结论云南西部地区宿主和媒介感染伯氏疏螺旋体基因型具有较高的多样性特征,存在多种致病基因型,给当地人群的健康造成一定危害,需加强防范。This study was aimed at understanding the genetic diversity of Borrelia burgdorferi infected by host animals and vector ticks in the western region of Yunnan province,to provide a basis to characterize epidemic strains or genotypes in this region.Genomic DNA templates were extracted from livestock,small mammals and tick specimens in western Yunnan province,and Borrelia DNA was identified through nested PCR amplification of the 5S-23S rRNA intergenic spacer region.Positive samples were further tested with 16S rRNA amplification and verified with the flagellin(FLA)gene.Five genotypes of B.burgdorferi sensu lato were detected in the host animals:B.afzelii,B.garinii,B.burgdorferi s.s.,B.japonica and B.valaisiana.Seven genotypes were found in vector ticks:B.afzelii,B.garinii,B.burgdorferi s.s.,B.japonica,B.valaisiana,B.sinica and Borrelia sp.Moreover,B.afzelii,B.garinii and B.burgerdorferi s.s.were found to be the dominant genotypes,accounting for 85.04%(256/301)of all positive samples.Intraspecific variations were observed in the same genotype of B.burgdorferi from different regions and specimen sources.B.burgdorferi detected in hosts and vectors in the same region showed high homology and close phylogenetic relationships.The high genetic diversity of B.burgdorferi in the hosts and vectors from western Yunnan,and their pathogenicity to humans,indicate potential health hazards to local residents.Prevention and control of Lyme borreliosis must urgently be strengthened in these regions.

关 键 词：云南西部 伯氏疏螺旋体 宿主动物 蜱 多样性 

分 类 号：R373.9[医药卫生—病原生物学]