玉米转录因子ZmNAC59调控植物抗盐性  

Transcription factor ZmNAC59 regulates plant salt resistance in Zea mays L.

作　　者：张宇涵 李星霖 刘丽君 傅竞也 王强[1] ZHANG Yuhan;LI Xinglin;LIU Lijun;FU Jingye;WANG Qiang(College of Agronomy,Sichuan Agricultural University,State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Chengdu 611130,China)

机构地区：[1]四川农业大学农学院,西南作物基因资源发掘与利用国家重点实验室,成都611130

出　　处：《西北植物学报》2024年第3期381-395,共15页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31971825)。

摘　　要：【目的】NAC转录因子家族是植物中研究较多的一类转录因子,在调控植物生长发育和响应非生物胁迫过程中起重要作用。玉米是三大粮食作物之一,其生长过程常会面临各种逆境胁迫,盐害被认为是限制作物生长和生产的主要环境因素之一。因此鉴定玉米抗盐基因,解析其抗盐机制对培育玉米抗逆品种具有重要意义。【方法】研究克隆了玉米转录因子ZmNAC59,并用生物信息学手段分析其保守结构域和系统进化关系,用实时荧光定量PCR方法分析该基因在NaCl和MeJA处理下的表达模式,并用稳定转基因体系将该基因异源表达拟南芥观察表型,同时还用病毒诱导沉默技术将该基因在玉米中沉默后进行盐处理表型观察,并进行酶活性检测。【结果】ZmNAC59能够被NaCl和MeJA诱导上调。病毒诱导沉默ZmNAC59后进行盐胁迫,沉默株系对盐胁迫更敏感,ROS积累更多;而ZmNAC59过表达拟南芥后,过表达株系在盐胁迫处理下存活率更高,活性氧积累更少,Na^(+)/K^(+)比率低,表明ZmNAC59作为盐胁迫中的正调控因子可以通过调节离子流动提高植物抗盐性。实时荧光定量PCR结果表明拟南芥过表达株系中Na^(+)、K^(+)转运相关的基因显著上调;在玉米原生质体中瞬时过表达ZmNAC59后进行盐处理发现ZmSOS1、ZmNHX1和ZmNHX7的表达显著上调,Dual-LUC实验表明ZmSOS1是Zm-NAC59的靶基因。【结论】研究表明ZmNAC59通过激活ZmSOS1启动子的活性调控其表达而促进盐胁迫下的离子转运,从而提高植物抗盐性,为玉米抗逆基因资源的筛选和品种培育提供了科学理论依据。[Objective]The NAC transcription factor family is a class of transcription factors that is widely studied in plants.It plays an important role in regulating plant growth and development and responding to abiotic stress.Maize is one of the three major food crops,which faces various adverse stresses in growth and development.Salt stress is one of the main environmental factors limiting crop growth and production.Therefore,it is of great significance to identify maize salt resistance genes and analyze their salt resistance mechanism for breeding stress-resistant maize varieties.[Methods]This study cloned the maize transcription factor ZmNAC59 and analyzed its conserved domain and phylogenetic relationship using bioinformatics methods.The expression pattern of the gene in leaves under NaCl and MeJA treatments was analyzed using the quantitative real-time PCR method.The stable transgenic system was used to het erologously express the gene in Arabidopsis thaliana for phenotype observation.At the same time,virus-induced silencing technology was used to silence the gene in maize,followed by phenotype observation un-der salt stress and enzyme activity detection.[Results]The qRT-PCR results showed that ZmNAC59 was up-regulated by NaCl and MeJA treatments.After virus-induced silencing of ZmNAC59,the silenced strains were more sensitive to salt stress and accumulated more ROS.After over expression of ZmNAC59 in A.thaliana,the over expression lines had higher survival rate,less accumulation of ROS,and lower Na^(+)/K^(+)ratios under salt stress,indicating that ZmNAC59,as a positive regulator of salt stress,can im-prove plant salt resistance by regulating ion homeostasis.qRT-PCR results showed that Na^(+)and K^(+)transport related genes were significantly up-regulated in A.thaliana overexpression lines.After transient expression of ZmNAC59 in maize protoplasts,the expression of ZmSOS1,ZmNHX1,and ZmNHX7 were significantly up-regulated after salt treatment.The Dual-LUC experiment showed that ZmSOS1 was the target gene of ZmNAC59.

关 键 词：玉米 转录因子 盐胁迫 离子转运 

分 类 号：Q946[生物学—植物学] S513[农业科学—作物学]