普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析  

Genome-wide identification of the PYL gene family in Luffa cylindrica and expression analysis in fruit development

作　　者：王玉倩 彭丽娟 刘建汀 温庆放[2,3] 钟凤林 朱海生[2,3] WANG Yuqian;PENG Lijuan;LIU Jianting;WEN Qingfang;ZHONG Fenglin;ZHU Haisheng(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Crops Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Fujian Key Laboratory of Vegetable Genetics and Breeding,Fuzhou 350013,China)

机构地区：[1]福建农林大学园艺学院,福州350002 [2]福建省农业科学院作物研究所,福州350013 [3]福建省蔬菜遗传育种重点实验室,福州350013

出　　处：《西北植物学报》2024年第3期408-420,共13页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建省属公益类科研院所基本科研专项(2021R1031001,2022R1031007);福建省自然科学基金项目(2021J01492);福建省农业科学院科技创新团队项目(CXTD2021003-1);国家大宗蔬菜产业体系福州综合试验站项目(CARS-23-G51);“5511”协同创新工程项目(XTCXGC2021003)。

摘　　要：【目的】探究普通丝瓜PYL基因在ABA处理下的表达模式,以期为深入研究其基因功能提供参考依据,也为分子抗性育种以及提高普通丝瓜品质和产量提供新思路。【方法】用生物信息学方法,对普通丝瓜PYL基因家族进行全基因组鉴定,染色体定位,以及基因结构、基因共线性、选择压力、进化亲缘关系和顺式作用元件分析,并通过qRT-PCR验证在不同浓度ABA下基因特征表达。【结果】在普通丝瓜全基因组中鉴定到11个LcPYL基因,CDS长度576~966 bp,编码蛋白均具有典型PYR_PYL_RCAR_like结构;共线性分析发现7对片段复制基因对,普通丝瓜PYL基因启动子含多个与植物激素相关的顺式作用元件,其成员的表达具有一定的组织特异性;普通丝瓜果实在不同浓度外源ABA处理下:2 mg/mL ABA处理LcPYLs表达无显著变化,4 mg/mL ABA处理LcPYL5、LcPYL7、LcPYL11显著上调表达,8 mg/mL ABA处理LcPYL1、LcPYL7开花当天显著表达。【结论】对普通丝瓜PYL家族成员进行了系统的鉴定与分析,明确其分子结构特征和表达模式,其中LcPYL1、LcPYL5、LcPYL7和LcPYL11可能是与内源ABA生物合成密切相关的关键基因。[Objective]Expression of PYL genes in Luffa cylindrica under ABA treatment was studied to provide a reference for gene function and to provide foundation for molecular breeding to improve the quality and yield of L.cylindrica.[Methods]Bioinformatics methods were used to analyze the distribution,chromosome location,gene structure,gene collinearity,selection pressure,evolutionary relationships,and cis-acting elements of the PYL gene family in L.cylindrica.Expression of the PYL gene family under different concentrations of ABA was verified by qRT-PCR.[Results]Eleven LcPYL genes were identified in the genome of L.cylindrica.LcPYL genes had CDSs of 576-966 bp with typical PYR_PYL_RCAR_like structures.Collinear analysis identified seven repeating gene pairs that were predicted to have similar functions.LcPYL promoters contained cis-acting elements associated with plant hormones,and the ex-pression of its members was tissue-specific.Expression of LcPYLs in L.cylindrica fruits did not change at 2 mg/mL ABA.However,the expression of LcPYL5,LcPYL7,and LcPYL11 was significantly in-creased at 4 mg/mL ABA,while LcPYL1 and LcPYL7 were expressed at the day of flowering at 8 mg/mL ABA.[Conclusion]Our findings suggest that LcPYL1,LcPYL5,LcPYL7,and LcPYL11 may be closely linked to endogenous ABA biosynthesis.

关 键 词：普通丝瓜 PYL基因家族 果实发育 外源ABA处理 表达分析 

分 类 号：Q786[生物学—分子生物学] S642.4[农业科学—蔬菜学]