辣椒CaNAC36的耐盐性分析  

Salt tolerance analysis of CaNAC36 in peppers

作　　者：龚成胜 郑雨晴 潘宝贵[1] 郭广君[1] 刁卫平[1] GONG Chengsheng;ZHENG Yuqing;PAN Baogui;GUO Guangjun;DIAO Weiping(Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;College of Horticulture,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,南京210014 [2]安徽农业大学园艺学院,合肥230036

出　　处：《西北植物学报》2024年第4期593-601,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：现代农业产业技术体系建设专项资金项目(CARS-23-G42);江苏省重点研发计划现代农业项目(BE2021377);江苏省高效园艺作物遗传改良重点实验室项目(ZD2022003);江苏省沿海集团盐碱地治理与地力提升科技揭榜挂帅项目(2022YHTDJB03)。

摘　　要：【目的】辣椒是中国种植面积最大的蔬菜作物,随着土地盐碱化问题日趋严重,探讨辣椒耐盐关键基因的功能,加强辣椒耐盐机制研究对促进辣椒产业可持续发展具有重要意义。【方法】研究组前期挖掘到辣椒盐胁迫响应相关的NAC转录因子家族基因CaNAC36,在此基础上,以耐盐辣椒PI201224和敏盐辣椒PI438643为供试品种,克隆获得CaNAC36全长gDNA和cDNA序列,荧光定量分析CaNAC36及可能互作基因在盐胁迫条件下组织表达情况,并结合生物信息学分析探究CaNAC36及其互作基因之间的潜在关系。【结果】CaNAC36序列在耐盐和敏盐材料中DNA和cDNA同源性分别为99.86%和100%;CaNAC36在耐盐材料根和茎组织中表现为诱导上调表达,在敏盐材料根和叶中表现为诱导下调表达;对可能与CaNAC36存在互作关系的48个基因的注释信息进行分析,发现跨膜蛋白、转运蛋白、水孔蛋白、氯离子通道蛋白、解毒蛋白等14个基因可能与CaNAC36存在功能互作。在PI201224和PI438643盐胁迫处理不同时间、不同组织中,5个相关基因(Capana08g002748、Capana00g004514、Capana09g000275、Capana07g001450、Capana02g001031)的表达呈显著差异;同时结合启动子分析发现CaNAC36及5个关联基因启动子域含有大量的逆境相关顺式作用元件。【结论】CaNAC36是辣椒响应盐胁迫的重要调节基因,其可能与其他基因相互作用而提高植株的耐盐性。[Objective]Pepper is the largest vegetable crop planted in China.With the land salinization becoming more and more serious,it is urgent to accelerate the functional study of key genes related to pepper salt tolerance.It is of great significance to strengthen the study of mechanisms underlying salt tolerance in peper to promote sustainable development of the pepper industry.[Methods]In the early stage of research,CaNAC36,a NAC transcription factor family gene related to salt stress response in peppers,was discovered.Based on this,the full-length gDNA and cDNA sequences of CaNAC36 were cloned using salttolerant pepper accession PI201224 and salt-sensitive pepper accession PI438643.The expression of CaNAC36 and its interacting genes in different tissues under salt stress was quantitatively analyzed by flu-orescence,and the potential relationship between CaNAC36 and its interacting genes was further explored by bioinformatics analysis.[Results]The gDNA and cDNA sequence homology of CaNAC36 in salt-toler-ant and salt-sensitive materials were 99.86%and 100%,respectively.CaNAC36 showed an up-expression in roots and stems in the salt-tolerant pepper,and a down-expression in roots and leaves in the salt-sensi-tive peper.Throgh analyzing the annotation information of 48 genes that may interact with CaNAC36,it was found that 14 genes were belonging to transmembrane proteins,transporter proteins,water porins,chloride channel proteins,detoxifying proteins,and other genes,which may have functional interaction with CaNAC36.Further analysis revealed that PI201224 and PI438643 exhibited significant differences in the expression of five related genes,inclding Capana08g002748,Capana00g004514,Capana09g000275,Capana07g001450,Capana02g001031,at different time points and tissues under salt stress.Meanwhile,the promoter region of CaNAC36 and five associated genes was found to contain a large number of stress-related cis-acting elements.[Conclusion]CaNAC36 was an important regulatory gene in pepper in re-sponse to salt stress,and might i

关 键 词：辣椒 CaNAC36 盐胁迫 基因互作 

分 类 号：S641.3[农业科学—蔬菜学]