一株异养硝化-好氧反硝化-积累磷的细菌Klebsiella pneumoniae A15的筛出及其特性研究  

Sieving and characterization of a heterotrophic nitrifying-aerobic denitrifying-phosphorus-accumulating bacterial strain,Klebsiella pneumoniae A15

作　　者：刘妍 袁林江 陈希 周旭红 陈雪娇 夏大朋 LIU Yan;YAN Lin-jiang;CHEN Xi;ZHOU Xu-hong;CHEN Xue-jiao;XIA Da-peng(Key Laboratory of Northwest Water Resources and Environmental Ecology,Ministry of Education,Key Laboratory of Environmental Engineering of Shaanxi Province,School of Environmental and Municipal Engineering,Xi’an University of Architecture and Technology,Xi’an 710055,China;School of Urban Planning and Municipal Engineering,Xi’an Polytechnic University,Xi’an 710048,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西省环境工程重点实验室,西北水资源与环境生态教育部重点实验室,陕西西安710055 [2]西安工程大学城市规划与市政工程学院,陕西西安710048

出　　处：《中国环境科学》2024年第4期2286-2296,共11页China Environmental Science

基　　金：国家自然科学基金资助项目(51078304,51278406);陕西省自然科学基金资助项目(2021JQ-690)。

摘　　要：利用以淀粉为唯一碳源的缺氧/好氧序批式系统从污水处理厂活性污泥中分离得到一株肺炎克雷伯氏杆菌A15(Klebsiella pneumoniae A15),该菌具有异养硝化-好氧反硝化和积聚磷能力.它的最佳生长条件:碳源为柠檬酸钠,C/N为30,P/N为0.2,pH为7.在最佳条件下,氨氮、亚硝酸盐和硝酸盐的最高去除率分别为96.27%、99.22%和100%;相应的去除速率分别为1.77、2.08和1.86mg/(L⋅h).该菌株对浓度为8mg/L的磷酸盐和1300mg/L的COD最高去除率分别为100%和95%.氮平衡分析发现该菌可以利用氨氮、硝氮以及亚硝氮产生气态氮,表现出优异的异养硝化和好氧反硝化活性.对菌株胞内磷和EPS中磷的分析结合DAPI染色发现有82%的磷储存在菌株细胞内并且以多聚磷酸盐形式储存,其余磷在EPS中.对该菌株的napA、nirS、nosZ和ppk基因的成功扩增也表明其好氧反硝化和积聚磷能力.本研究展示了一株在脱氮除磷系统中具有特定功能的细菌.A strain of Klebsiella pneumoniae A15 with heterotrophic nitrification-aerobic denitrification and phosphorus accumulation was isolated from activated sludge of a wastewater treatment plant using an anoxic/aerobic sequencing batch system with starch as the sole carbon source.Its optimal growth conditions:carbon source was sodium citrate,C/N was 30,P/N was 0.2,and pH was 7.Under the optimal conditions,the maximum removal rates of ammonia nitrogen,nitrite,and nitrate were 96.27%,99.22%,and 100%,respectively;and the corresponding rates of removal were 1.77,2.08 and 1.86mg/(L⋅h).The strain was effective in removing phosphorus at concentrations of 8mg/L and 1300mg/L COD with maximum removal rates of 100% and 95%,respectively.Nitrogen balance analysis revealed that the bacterium could utilize ammonia,nitrate and nitrite nitrogen to produce gaseous nitrogen,and showed excellent heterotrophic nitrification and aerobic denitrification activities.Analysis of intracellular phosphorus and phosphorus in the EPS of the strain combined with DAPI staining revealed that 82% of the phosphorus was stored in the cells of the strain and in the form of polyphosphates,with the remaining phosphorus in the EPS.Successful amplification of napA,nirS,nosZ and ppk genes of the strain also indicated its aerobic denitrification and phosphorus accumulation capacity.The present study demonstrates a bacterial strain with specific functions in the denitrification and phosphorus removal system.

关 键 词：异养硝化 除磷菌 DAPI染色 聚磷颗粒 

分 类 号：X703.5[环境科学与工程—环境工程]