高效液相色谱测定温肾暖脾颗粒中补骨脂素和异补骨脂素含量  

Determination of the content of psoralen and isopsoralen in Wenshen Nuanpi Granules by high performance liquid chromatography

作　　者：邓永志 李杰[2] 赵丹[2] 杨薇 张文 何蕊[4] 位鸿[4] 刘洋[4] DENG Yongzhi;LI Jie;ZHAO Dan;YANG Wei;ZHANG Wen;HE Rui;WEI Hong;LIU Yang(The Third Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130117,China;Changchun University of Chinese Medicine,Changchun 130117,China;Changchun Anti-Cancer Pharmaceutical Research Institution,Changchun 130022,China;The Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China)

机构地区：[1]长春中医药大学附属第三临床医院,长春130117 [2]长春中医药大学,长春130117 [3]长春市抗癌药物研究所,长春130022 [4]长春中医药大学附属医院,长春130021

出　　处：《长春中医药大学学报》2024年第4期409-414,共6页Journal of Changchun University of Chinese Medicine

基　　金：吉林省科技发展计划项目(20210204075YY)。

摘　　要：目的建立温肾暖脾颗粒补骨脂素、异补骨脂素的含量测定方法,为质量控制提供实验依据。方法以十八烷基硅烷键合硅胶为填充剂;流动相:甲醇-水(40:60);检测波长246 nm;结果峰面积和质量浓度之间线性关系良好,补骨脂素R^(2)=0.9992,异补骨脂素R^(2)=0.9993;精密度、稳定性均符合要求;补骨脂素平均回收率为98.25%,RSD为0.89%,异补骨脂素平均回收率为95.90%,RSD为1.18%。结论本试验研究并建立了高效液相色谱(high performance liquid chromatography,HPLC)补骨脂素、异补骨脂素的含量测定方法,可以作为温肾暖脾颗粒质量控制的依据。Objective To establish a method for determining the contents of psoralen and isopsoralen in Wenshen Nuanpi Granules in order to provide experimental basis for quality control of Wenshen Nuanpi Granules.Methods Octadecylsilane bonded silica gel was used as filling agent.The mobile phase was methanol:water(40:60 v/v).The detected wavelength was 246 nm.Results Linear-relationship between peak area and mass concentration was good for psoralen(r^(2)=0.9992)and isopsoralen(r^(2)=0.9993);The precision and stability were in accordance with the requirements;The average recovery rate of psoralen was 98.25% with an RSD of 0.89%,and the average recovery rate of isopsoralen was 95.90%with an RSD of 1.18%.Conclusion In this experiment,the high performance liquid chromatography method for determining the content of psoralen and isopsoralen is investigated and established,which can be used as the basis for the quality control of Wenshen Nuanpi Granules.

关 键 词：温肾暖脾颗粒 高效液相色谱 补骨脂素 异补骨脂素 

分 类 号：R282[医药卫生—中药学]