菜豆炭疽病病原鉴定及研究  

Identification and study on the pathogen of common bean anthracnose

作　　者：王振磊 刘大军[1] 冯国军[1] 杨晓旭 刘畅[1] 闫志山[1] WANG Zhenlei;LIU Dajun;FENG Guojun;YANG Xiaoxu;LIU Chang;YAN Zhishan(College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin 150086,China)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150086

出　　处：《黑龙江大学自然科学学报》2024年第1期70-78,共9页Journal of Natural Science of Heilongjiang University

基　　金：黑龙江省“百千万”工程科技重大专项(2019ZX16B02)。

摘　　要：为了确定黑龙江省哈尔滨市呼兰区导致菜豆炭疽病的病原菌种类,通过分离培养得到了菌株WZL01,通过形态学和分子生物学的综合鉴定确定该菌株为炭疽菌(Colletotrichum spaethianum,C.spaethianum)。利用菌丝生长法和镜检法对该菌株进行了生物学特性研究,该菌株适宜在25~30℃、pH=6~8及黑暗条件下生长,适宜的培养基为马铃薯葡萄糖琼脂(Potato dextrose ager,PDA)和菜豆叶培养基,适宜的碳源为麦芽糖,适宜的氮源为硫酸铵。在室内进行了杀菌剂毒力测定,多菌灵等8种杀菌剂对该菌都有抑制作用,其中40%苯醚甲环唑和50%多菌灵的半效应浓度(Median effect concentration,EC50)值较小,分别为0.0318和0.4813,抑制效果较好。本研究明确了黑龙江省哈尔滨市呼兰区菜豆种植区菜豆炭疽病的病原菌、生物学特性和适用化学药剂,为今后当地抗病品种筛选及田间防治奠定了基础。In order to confirm the pathogenic species leading to common bean anthracnose in the region of Hulan district,Harbin city,Heilongjiang province,the strain WZL01 was obtained by isolation and culture.Through the comprehensive identification of morphology and molecular biology,the strain was determined to be Colletotrichum spaethianum(C.spaethianum).The biological characteristics of the strain by mycelial growth method and microscopic examination method were studied.The results show that the strain is suitable for growth in 25~30℃,pH=6~8,dark conditions,the appropriate medium is potato dextrose ager(PDA)and common bean leaf medium,the appropriate carbon source is maltose,and the nitrogen source is ammonium sulfate.The fungicide virulence test is conducted in the room,and the results show that 8 fungicides such as carbendazim,and the median effect concentration(EC_(50))values of 40%difenoconazole and 50%carbendazim are 0.0318 and 0.4813,respectively,showing better inhibitory effects.The pathogen,biological characteristics and applicable chemical agents of common bean anthracnose in the common bean planting area of Hulan District,Harbin City,Heilongjiang Province are clarified,which lays a research foundation for the future screening of local disease-resistant varieties and field control.

关 键 词：菜豆 炭疽病 病原鉴定 生物学特性 杀菌剂 

分 类 号：S643.1[农业科学—蔬菜学]