多年宿根甘蔗的转录组分析及差异基因挖掘  

Transcriptome Analysis and Differential Gene Mining of Perennial-root Sugarcane

作　　者：程琴[1] 李佳慧 谭秦亮[1] 宋奇琦 朱鹏锦[1] 欧克纬[1] 农泽梅 卢业飞[1] 吕平[1] 周全光[1] 庞新华[1] 彭欣怡 CHENG Qin;LI Jiahui;TAN Qinliang;SONG Qiqi;ZHU Pengjin;OU Kewei;NONG Zemei;LU Yefei;LV Ping;ZHOU Quanguang;PANG Xinhua;PENG Xinyi(Guangxi Institute of Subtropical Crops,Nanning 530001,China)

机构地区：[1]广西壮族自治区亚热带作物研究所,南宁530001

出　　处：《中国生物工程杂志》2024年第2期25-38,共14页China Biotechnology

基　　金：广西科技计划(桂科AB23026083、桂科AA22117002-6);广西农业科学院基本科研业务专项(桂农科2021YT151)资助项目。

摘　　要：甘蔗(Saccharum hybirds)宿根性直接关系到甘蔗生产成本和种植效益,品种、环境和栽培措施均会影响甘蔗宿根能力,但品种的种性是影响宿根性最关键因素。国内外从分子生物学层面解释甘蔗宿根性差异的文献鲜见报道,从转录水平分析不同宿根年限GR2号和ROC22号基因表达的差异。结果表明:转录组测序共得到100558条转录本和25582条Unigene。获得53790条Unigene的注释结果,分别在NR、Swiss-Prot、KEGG、COG、KOG、GO和Pfam数据库进行比对。GO功能注释共分成三大类,51小类,6年宿根蔗注释到1029个差异基因,3年宿根蔗注释到3391个差异基因。主要KEGG代谢通路有8条,分别涉及植物激素信号转导,淀粉和蔗糖代谢,甘氨酸、丝氨酸和苏氨酸代谢,苯丙素生物合成,同源重组,DNA复制,错配修复及植物病原体相互作用。筛选出脱落酸(ABA)相关差异基因6个,分别为脱落酸不敏感蛋白2基因(ABI2)、bZIP转录因子超家族蛋白、碱性亮氨酸拉链型转录因子基因(ABI5)、aba响应元件结合因子1基因(ABF1)、G-box结合因子基因(GBFs)、aba响应元件结合因子基因(ABF)。以上差异基因将作为后期进行基因表达和功能分析的参考基因,并联合蛋白质组学深入分析影响甘蔗宿根性的分子机制。Sugarcane ratooning ability is directly related to its production cost and cultivation efficiency.Sugarcane ratooning productivity is influenced by variety,environment and cultivation measures,but variety is the most critical factor.There are few reports explaining the differences in sugarcane ratooning ability at the molecular biological level both domestically and internationally.In this paper,transcriptome sequencing was used to analyze the differences in gene expression between sugarcane GR2 and ROC22 of different root years.The results showed that 100558 transcripts and 25582 unigenes were obtained by transcriptome sequencing.By functional annotation of unigenes,including comparison with NR,Swiss-Prot,KEGG,COG,KOG,GO and Pfam databases,a total of 53790 unigene annotation results were obtained.The GO functional annotation was divided into three categories and 51 subcategories.A total of 1029 differential genes were annotated in six-year-old sugarcane,and 3391 differential genes were annotated in three-year-old sugarcane.There are eight major KEGG metabolic pathways,which are mainly involved in plant hormone signal transduction,starch and sucrose metabolism,glycine,serine and threonine metabolism,phenylpropanoid biosynthesis,homologous recombination,DNA replication,mismatch repair and plant pathogen interaction.Six abscisic acid-related differentially identified genes are:abscisic acid-insensitive 5-like protein 2(ABI2),putative bZIP transcription factor superfamily protein,protein abscisic acid-insensitive 5(ABI5),aba responsive element-binding factor 1(partial ABF1),G-box binding factor 4(GBFs),and aba responsive element-binding factor 1(ABF).The above differential genes will serve as reference genes for our later gene expression and function analysis,and further analyze the molecular mechanism affecting sugarcane ratooning ability by combining proteomics.

关 键 词：甘蔗 宿根性 转录组 脱落酸 

分 类 号：Q78[生物学—分子生物学]