兴安落叶松成熟合子胚高效离体再生体系的建立  

Establishment of an Efficient in Vitro Regeneration System for Mature Zygotic Embryos in Larix gmelinii

作　　者：张旭婷 王佳秀 刘槟 张燕霞 尚晓蕊 单金元 李国婧[1] 王瑞刚[1] ZHANG Xuting;WANG Jiaxiu;LIU Bin;ZHANG Yanxia;SHANG Xiaorui;SHAN Jinyuan;LI Guojing;WANG Ruigang(Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学旱寒区植物逆境适应与遗传修饰改良自治区重点实验室,呼和浩特010018

出　　处：《中国生物工程杂志》2024年第2期85-93,共9页China Biotechnology

基　　金：国家转基因生物新品种培育专项(2018ZX08020-003);呼和浩特市重大科技专项(2022-社-重-1-3);内蒙古自治区高校创新团队(NMGIRT2222)资助项目。

摘　　要：以兴安落叶松成熟合子胚为外植体,开展落叶松离体器官发生的探究。实验选用多种培养基,以6-BA和2,4-D等生长调节剂进行愈伤组织和不定芽诱导,进行了不定芽伸长和生根诱导。结果表明:愈伤组织在1/2 MS+2 mg/L 2,4-D培养基中诱导率最高,愈伤组织诱导率为91.11%;不定芽的诱导培养基为1/2 MS+1 mg/L 6-BA+0.07 mg/L 2,4-D,不定芽诱导率为87.78%;不定芽伸长培养基为MS+0.20 mg/L 6-BA+0.01 mg/L 2,4-D+1 mg/L活性炭,不定芽伸长率达到48.33%;不定根生根培养基为1/2 MS+1 mg/L IBA,不定根诱导率达到36.67%;落叶松的再生苗移植成活率已达到了100%。上述研究成果为落叶松组培器官再生技术和进一步稳定遗传改良工作提供了良好的理论基础和实验依据。The mature zygotic embryos of Larix gmelinii were used as explants to study the isolated organogenesis of larch.The experimental design used different medium,including 6-BA and 2,4-D to induce healing and adventitious shoots,elongation and rooting.The results showed that the best callus induction medium was 1/2 MS+2 mg/L 2,4-D,and callus induction rate was 91.11%.Induction medium for adventitious budding was 1/2 MS+1 mg/L 6-BA+0.07 mg/L 2,4-D,and the adventitious shoot induction rate was 87.78%.The indefinite bud elongation medium was MS+0.20 mg/L 6-BA+0.01 mg/L 2,4-D+1 mg/L activated carbon,and the elongation rate of adventitious buds reached 48.33%.Adventitious root rooting medium was 1/2 MS+1 mg/L IBA,and the induction rate of indefinite root reached 36.67%.The survival rate of transplanted seedlings was 100%.The above studies provide a good theoretical and practical basis for larch tissue culture organogenesis and further research on stable genetic transformation.

关 键 词：兴安落叶松 成熟合子胚 再生体系 

分 类 号：Q813[生物学—生物工程]