子痫前期患者胎盘组织中缺氧诱导因子-1α表达及其与血管生成的关系  

Expression of Hypoxia Inducible Factor-1αin Placenta of Preeclampsia Patients and its Relationship with Angiogenesis

作　　者：李静亚 蔡素霞 张广裕 LI Jingya;CAI Suxia;ZHANG Guangyu(Department of Pathology,Luoyang Maternal and Child Health Hospital,Luoyang 471099,China;Delivery Room,Luoyang Maternal and Child Health Hospital,Luoyang 471099,China)

机构地区：[1]洛阳市妇幼保健院病理科,河南洛阳471099 [2]洛阳市妇幼保健院产房,河南洛阳471099

出　　处：《河南医学研究》2024年第7期1287-1290,共4页Henan Medical Research

摘　　要：目的分析子痫前期患者胎盘组织中缺氧诱导因子-1α(HIF-1α)表达及其与血管生成的关系。方法前瞻性选取2019年1月至2023年1月在洛阳市妇幼保健院接受治疗并成功分娩的80例子痫前期患者作为研究对象,其中轻症患者48例(轻症组),重症患者32例(重症组),另外选取50例同期健康孕妇作为对照组。于全部孕妇分娩后取其全层胎盘组织,检测并记录胎盘组织微血管密度,检测HIF-1αmRNA表达水平,计算HIF-1α阳性表达率。测定孕产妇的活化部分凝血活酶时间(APTT)、血浆凝血酶原时间(PT)、纤维蛋白原(Fib)等凝血相关指标水平。分析HIF-1α相对表达量和胎盘组织中血管生成的关系。结果重症组胎盘组织中微血管密度低于轻症组和对照组,且轻症组低于对照组(P<0.05)。重症组HIF-1αmRNA、Fib水平高于轻症组和对照组,APTT、PT低于轻症组和对照组,轻症组HIF-1αmRNA、Fib水平高于对照组,APTT、PT低于对照组(P<0.05)。重症组HIF-1α总阳性表达率高于轻症组与对照组,轻症组高于对照组。子痫前期患者胎盘组织中HIF-1α相对表达量与血管生成呈负相关(r<0,P<0.05)。结论HIF-1α在子痫前期患者的胎盘组织中呈异常过表达,且表达水平和胎盘组织血管生成呈负相关。Objective To analyze the relationship between the expression of hypoxia inducible factor 1α(HIF-1α)and angiogenesis in placenta of patients with preeclampsia.Methods A total of 80 cases of preeclampsia who were treated in Luoyang Maternal and Child Health Hospital from January 2019 to January 2023 and delivered successfully were prospectively selected as the research objects,including 48 cases of mild patients(mild group)and 32 cases of severe patients(severe group),and 50 cases of healthy pregnant women in the same period were selected as the control group.After delivery,all pregnant women were taken from the entire layer of placental tissue,and the microvessel density of placental tissue was measured and recorded.The expression level of HIF-1αmRNA was measured,and the positive expression rate of HIF-1αwas calculated.The levels of activated partial thromboplastin time(APTT),plasma Prothrombin time(PT),fibrinogen(Fib)and other coagulation related indicators in pregnant and lying in women were measured.The relationship between the relative expression of HIF-1αand angiogenesis in placental tissue was analyzed.Results The microvascular density in the placental tissue of the severe group was lower than mild group and the control group,and the mild group was lower than the control group(P<0.05).The levels of HIF-1αmRNA and Fib in the severe group were higher than those in the mild group and control group,while APTT and PT were lower than those in the mild group and control group,the levels of HIF-1αmRNA and Fib in the mild group were higher than those in the control group,while APTT and PT were lower than control group(P<0.05).The total positive expression rate of HIF-1αin the severe group was higher than mild and control groups,and the mild group was higher than control group(P<0.05).The relative expression level of HIF-1αin placental tissue of preeclampsia patients was negatively correlated with angiogenesis(r<0,P<0.05).Conclusion HIF-1αis abnormally overexpressed in placental tissue of preeclampsia patien

关 键 词：子痫前期 胎盘组织 缺氧诱导因子HIF-1Α 血管生成 凝血指标 

分 类 号：R714.25[医药卫生—妇产科学]