碳酸盐碱度对大鳞副泥鳅胚胎发育和仔鱼活力的影响  

Effect of carbonate alkalinity on embryonic development and larval vitality of Pramisgurnus dabryanus

作　　者：许海鑫 朱洪赓 张家敏 朱明 王海华[3] 陈建华 XU Haixin;ZHU Honggeng;ZHANG Jiamin;ZHU Ming;WANG Haihua;CHEN Jianhua(Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyugang 222005,China;Lianyungang Yinhe Loach Co.,Ltd.,Lianyugang 222142,China;Nanchang Key Laboratory of Special Aquatic Breeding and Healthy Aquaculture,Jiangxi Fisheries Research Institute,Nanchang 330039,China)

机构地区：[1]江苏海洋大学/江苏省海洋生物技术重点实验室,江苏连云港222005 [2]连云港市银合泥鳅有限公司,江苏连云港222142 [3]江西省水产科学研究所/南昌市特种水产繁育与健康养殖重点实验室,江西南昌330039

出　　处：《南方水产科学》2024年第2期56-62,共7页South China Fisheries Science

基　　金：江苏省现代农机装备与技术示范推广项目(NJ2021-05);江西省主要学科学术和技术带头人培养计划领军人才项目(20225BCJ2201);连云港市科技成果转化揭榜挂帅专项资金项目(CA202202)。

摘　　要：为探究大鳞副泥鳅(Paramisgurnus dabryanus)胚胎及仔鱼对碳酸盐碱度的适应性,将发育至囊胚期的胚胎分别放置于碳酸盐碱度为0、10、20、30和40 mmol·L^(-1)的水体中培养,记录胚胎的孵化率、孵化时间,统计仔鱼的畸形率、成活率、特定成长率及卵黄囊吸收率,并测定初孵仔鱼的不投饵存活系数(Survival activity index,SAI)。结果显示:1)碳酸盐碱度为10 mmol·L^(-1)时,胚胎孵化率最高,但与对照组差异不显著(P>0.05),碳酸盐碱度在10~20 mmol·L^(-1)时,孵化时间显著短于对照组(P<0.05);2)初孵仔鱼的畸形率随碳酸盐碱度的增加而逐渐升高,3日龄(Days after hatching,dah)仔鱼成活率则随碳酸盐碱度的增加逐渐降低;3)初孵仔鱼全长随碳酸盐碱度的增加先下降后上升,而3 dah仔鱼全长随碳酸盐碱度的增加先上升后下降,其特定生长率及卵黄囊吸收率均呈先升后降的趋势;4)随着碳酸盐碱度的增加,仔鱼SAI先升后降,其中10 mmol·L^(-1)组达到最大值。结果表明,碳酸盐碱度在0~10 mmol·L^(-1)范围内适宜大鳞副泥鳅的胚胎发育及仔鱼生长。To investigate the adaptation of the embryos and larvae of Paramisgurnus dabryanus to carbonate alkalinity,we cultured the embryos which had developed to the blastocyst stage in water at carbonate alkalinities of 0,10,20,30 and 40 mmol·L^(-1),so as to investigate the hatching period,hatching rate,abnormality rate,survival rate,specific growth rate(SGR),yolk sac absorption and survival activity index(SAI).The results show that:1)The embryo hatching rate was highest at carbonate alkalinity of 10 mmol·L^(-1) without significant difference compared with that of the control group(P>0.05).The hatching period was significantly shorter than that of the control group at carbonate alkalinities of 10 and 20 mmol·L^(-1)(P<0.05).2)The abnormality rate of the newly hatched larvae ascended gradually with the increase of carbonate alkalinity,while the survival rate of larvae of 3 dah(Days after hatching)gradually decreased.3)The total length of newly hatched larvae decreased and then increased,while the total length of larvae of 3 dah increased and then decreased with the increase of carbonate alkalinity.The SGR and yolk sac absorption rate showed an increasing and then decreasing tendency.4)The larval SAI first increased and then decreased with the increase of carbonate alkalinity and reached the maximum in 10 mmol·L^(-1) group.It is indicated that the optimal carbonate alkalinity for the embryonic development and larval growth of P.dableanus is 0-10 mmol·L^(-1).

关 键 词：大鳞副泥鳅 碳酸盐碱度 仔鱼 胚胎发育 不投饵存活系数 

分 类 号：S917.4[农业科学—水产科学]