长鳍光唇鱼(Acrossocheiluslongipinnis)人工繁殖和早期发育研究  

Study on artificial propagation and early development of Acrossocheilus longipinnis

作　　者：王岳松 徐林 杨洋 程睿 王崇[1] 何大江 黎国樑 马海涛 毕建启 陈锋[1] 万正平 张志明 WANG Yuesong;XU Lin;YANG Yang;CHENG Rui;WANG Chong;HE Dajiang;LI Guoliang;MA Haitao;BI Jianqi;CHEN Feng;WAN Zhengping;ZHANG Zhiming(Institute of Hydroecology,Ministry of Water Resources and Chinese Academy of Sciences/Key Laboratory of Ecological Impacts of Hydraulic Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources,Wuhan 430079,China;Guangxi Datengxia Gorge Water Conservancy Development Co.,Ltd.,Nanning 530000,China;Guangzhou Pearl River Water Resources Protection Technology Development Co.,Ltd.,Guangzhou 510000,China)

机构地区：[1]水利部中国科学院水工程生态研究所/水利部水工程生态效应与生态修复重点实验室,湖北武汉430079 [2]广西大藤峡水利枢纽开发有限责任公司,广西南宁530000 [3]广州珠江水资源保护科技发展有限公司,广东广州510000

出　　处：《南方水产科学》2024年第2期63-72,共10页South China Fisheries Science

基　　金：科技基础资源调查专项(2019FY1011900);来宾市红水河珍稀鱼类增殖保护站运行管理(DHB2022013);水电工程鱼类栖息地保护监测和信息化管理关键技术研究与示范应用(DJ-ZDXM-2020-02)。

摘　　要：长鳍光唇鱼(Acrossocheilus longipinnis)是红水河珍稀特有鱼类,也是当地的重要经济物种。为实现长鳍光唇鱼苗种规模化生产,并为其增殖放流提供技术指导,对人工驯养的长鳍光唇鱼亲鱼进行强化培育和催产,采用干法授精获得受精卵,并观察其胚胎及仔鱼的发育过程。连续进行了4次人工催产,催产率为(68.95±13.26)%、受精率为(68.50±19.82)%、孵化率为(80.00±10.23)%。长鳍光唇鱼受精卵为沉性卵,具弱黏性,呈金黄色,卵径为(2.42±0.06)mm,吸水膨胀后为(3.03±0.08)mm。胚胎发育过程历经胚盘形成期、卵裂期、囊胚期、原肠期、神经胚期、器官形成期和孵化期7个阶段、28个发育时期。水温22~24℃时,经66~74 h孵化出膜,积温为1501.13℃·h。初孵仔鱼平均全长为(8.33±0.23)mm,肌节34对,4日龄仔鱼形成5条横斑雏形。卵黄囊期历时8 d,全长特定生长率为4.13%·d^(-1);仔鱼期共历时21 d,全长特定生长率为2.70%·d^(-1)。研究结果对保护红水河鱼类资源及其多样性具有重要意义。Acrossocheilus longipinnis is a rare fish and an economically important species in the Hongshui River.To achieve large-scale production of A.longipinis fry and provide technical guidance for its proliferation and release,artificially domesticated A.longipinnis were intensively cultivated and induced to produce fertilized eggs by dry fertilization.The embryonic and larval development was observed.Four times of artificial inductions were performed.The oxytocin rate was(68.95±13.26)%,the fertilization rate was(68.50±19.82)%,and the hatching rate was(80.00±10.23)%.The fertilized eggs of the A.longipinnis were sedimentary,weakly viscous and golden in color.The egg diameter of the yolk was(2.42±0.06)mm,and the expansion of water absorption was(3.03±0.08)mm.The embryonic development process went through a total of 7 stages and 28 periods including blastogenesis stage,cleavage stage,blastocyst stage,gastrum stage,neuroembryo stage,organogenesis stage and hatching stage.The membrane was hatched after 66-74 h at the water temperature of 22-24℃,with an accumulated temperature of 1501.13℃·h.The total length of the newly hatched larvae was(8.33±0.23)mm,with 34 pairs of sarcotomes.At 4 days after hatching,the larvae formed 5 transversely spotted embryonic prototypes.The yolk sac stage lasted for 8 d,with a total length special growth rate of 4.13%·d^(-1).The larvae stage lasted for 21 d,with a special growth rate of total length of 2.70%·d^(-1).The results are important for protecting A.longipinnis resources and diversity of the Hongshui River.

关 键 词：长鳍光唇鱼 人工繁殖 胚胎发育 仔鱼生长 红水河 

分 类 号：S961.2[农业科学—水产养殖]