肉苁蓉骨靶向纳米粒促进大鼠骨缺损修复的实验研究  

Experimental Study on Cistanche deserticola Bone Targeting Nanoparticles Promoting Bone Defect Repair in Rats

作　　者：储建函 梁子聪[1] 陈其宽 CHU Jian-han;LIANG Zi-cong;CHEN Qi-kuan(Basic medicine department of Qiannan Medical College For Nationalities,Duyun,558013,Guizhou;Qiannan Hospital of Traditional Chinese Medicine(The Second Affiliated Hospital of Qiannan Medical College For Nationalities),Duyun,558000,Guizhou)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558013 [2]黔南州中医医院(黔南民族医学高等专科学校第二附属医院),贵州都匀558000

出　　处：《黔南民族医专学报》2024年第1期4-7,共4页Journal of Qiannan Medical College for Nationalities

基　　金：2019年度国家重点研发计划“中医药现代化”重点专项(2019YFC1712500);黔南民族医学高等专科学校科研基金项目(QNYZ201815、QNYZ202210);黔南民族医学高等专科学校大学生创新项目(QNYZ202204)。

摘　　要：目的:观察肉苁蓉骨靶向纳米粒促进大鼠骨缺损的修复作用。方法:选取清洁级雄性SD大鼠40只,采用随机数字表法随机分成4组,分别设立正常对照组、四环素纳米粒(TC-PLGANP)组、肉苁蓉纳米粒(CH/PLGANP)组、肉苁蓉骨靶向纳米粒(CH/TC-PLGANP)组,每组10只。除正常对照组外,每组按照相应方法建立大鼠股骨缺损模型。药物干预后,应用蛋白印迹法和免疫组化法检测骨组织中的FGF-2、VEGF、BMP-2表达量。结果:与正常对照组比较,TC-PLGANP组、CH/PLGANP组、CH/TC-PLGANP组大鼠骨组织FGF-2、VEGF、BMP-2的蛋白相对表达量、阳性颗粒相对表达量明显升高(P<0.05);与TC-PLGANP组比较,CH/PLGANP组、CH/TC-PLGANP组大鼠骨组织FGF-2、VEGF、BMP-2的蛋白相对表达量、阳性颗粒相对表达量明显升高(P<0.05);与CH/PLGANP组比较,CH/TC-PLGANP组大鼠骨组织FGF-2、VEGF、BMP-2的蛋白相对表达量、阳性颗粒相对表达量明显升高(P<0.05)。结论:肉苁蓉骨靶向制剂相较肉苁蓉普通制剂而言,骨组织中促进骨修复因子表达量更高,有利于骨缺损的修复。Objective:To observe the effect of Cistanche deserticola bone targeted nanoparticles on promoting the repair of bone defects in rats.Methods:Forty clean male SD rats were randomly divided into four groups using a random number table method,namely a normal control group,tetracycline nanoparticles(TC-PLGANP)group,Cistanche deserticola nanoparticles(CH/PLGANP)group,and Cistanche deserticola bone targeted nanoparticles(CH/TC-PLGANP)group,with 10 rats in each group.Except for the normal control group,each group was established using a corresponding method to establish a rat femoral defect model.After drug intervention,western blot and immunohistochemical methods were used to detect the expression levels of FGF-2,VEGF,and BMP-2 in bone tissues.Results:Compared with the normal control group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the TC-PLGANP group,CH/PLGANP group,and CH/TC-PLGANP group significantly increased(P<0.05).Compared with the TC-PLGANP group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the CH/PLGANP group and CH/TC-PLGANP group significantly increased(P<0.05).Compared with the CH/PLGANP group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the CH/TC-PLGANP group significantly increased(P<0.05).Conclusion:Compared with ordinary preparations of Cistanche deserticola,bone targeted preparations of Cistanche deserticola have higher expression levels of bone repair factors in bone tissues,which is beneficial to the repair of bone defects.

关 键 词：骨靶向 肉苁蓉 骨修复 

分 类 号：R285.5[医药卫生—中药学]