猪伪狂犬病毒抗体检测方法的研究进展  

Research Progress on the Antibody Detection Methods for Porcine Pseudorabies Virus

作　　者：卢立康 许楷惠 黄元 蔡汝建 王晓虎 陈晶 向华 LU Likang;XU Kaihui;HUANG Yuan;CAI Rujian;WANG Xiaohu;CHEN Jing;XIANG Hua(Zhongkai University of Agriculture and Engineering,Guangzhou Guangdong 510225;Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640)

机构地区：[1]仲恺农业工程学院,广东广州510225 [2]广东省农业科学院动物卫生研究所,广东广州510640

出　　处：《广东畜牧兽医科技》2024年第2期52-56,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省重点领域研发计划(2020B0202080004,2019B020211003,2019B020217002);广东省现代农业产业技术体系生猪创新团队项目。

摘　　要：伪狂犬病,也称阿氏病(Aujeszky'sdisease),是由伪狂犬病毒(PRV)引起的一种高度传染性疾病。在没有特定宿主的情况下,伪狂犬病毒可以侵入多种哺乳动物,包括猪、牛、羊等,从而导致易感动物发生严重的临床症状和急性死亡。我国于20世纪50年代首次报道猪伪狂犬病,且自2011年以来,部分地区出现新的猪伪狂犬病变异株,致使养猪业遭受严重的经济损失。尽管科学家们一直在致力于诊断方法的设计和相关疫苗的开发,但伪狂犬病仍在养猪业广泛存在,近些年发现其对人类的健康也存在着潜在威胁,从而引起了世界范围内的强烈关注。目前我国的猪场普遍实行伪狂犬疫苗免疫,因此检测免疫后疫苗产生的抗体水平至关重要。本文总结目前对PRV的抗体检测方法,针对其优缺点进行对比分析,以对猪场临床上选择抗体检测方法提供参考。Pseudorabies,also called Aujeszky's disease,is a highly contagious disease caused by pseudorabies virus(PRV).In the absence of a specific host,pseudorabies virus can invade the body of a variety of mammals,including pigs,cattle,sheep,etc.,which leads to severe clinical symptoms and acute death in susceptible animals.The existence of porcine pseudorabies was first reported in China in the 1950s,and since 2011,new porcine pseudorabies variants have emerged in some areas,resulting in serious economic losses to the pig industry.Although scientists have been working on the design of diagnostic methods and the development of related vaccines,pseudorabies is still widespread in the pig industry,and its discovery in recent years that it also has potential problems for human health has aroused strong concern in the world.At present,pseudorabies vaccine is widely immunized in pig farms in China,so it is very important to detect the antibody level produced by the vaccine after immunization.In this paper,the current antibody detection methods for PRV will be summarized,and their advantages and disadvantages will be compared and analyzed in order to provide a reference for the selection of antibody detection methods on pig farms.

关 键 词：伪狂犬病毒 抗体检测 病毒中和试验 ELISA 

分 类 号：S852.65[农业科学—基础兽医学]