XJ-21菌株在胍胶压裂液体系中破胶驱油的复合性能  

Compound performance of XJ-21 strain in guar gum fracturing fluid system

作　　者：翟怀建 董景锋 张凤娟 尹剑宇 孙秀鹏 赵静 ZHAI Huaijian;DONG Jingfeng;ZHANG Fengjuan;YIN Jianyu;SUN Xiupeng;ZHAO Jing(Research Institute of Engineering Technology,Xinjiang Oilfield Company,PetroChina,Kelamayi 834000,Xinjiang,China;Dalian Chivy Biotechnology Co.,Ltd.,Dalian 116000,Liaoning,China)

机构地区：[1]中国石油新疆油田分公司工程技术研究院,新疆克拉玛依834000 [2]大连知微生物科技有限公司,辽宁大连116000

出　　处：《精细化工》2024年第4期911-919,共9页Fine Chemicals

基　　金：大连市重点研发科技计划项目(2022YF12GX058);大连市科技人才创新支持计划(青年科技之星)项目(2022RQ063)。

摘　　要：在储层压裂改造过程中,胍胶压裂液体系低温破胶难,储层改造效果差,是新疆油田油藏开发亟待解决的关键技术难题。针对这一技术难题,将微生物强化采油技术应用于压裂液体系中,从新疆油田吉7井区烧房沟组采集到油泥,通过对样品中微生物的富集培养及分离,筛选得到一株能够高产生物表面活性剂的菌株,将其命名为XJ-21。结合菌落形态观察、革兰氏染色及16S rRNA序列分析结果,XJ-21菌株被鉴定为枯草芽孢杆菌(Bacillussubitlis)。FTIR分析确定XJ-21代谢产物中的生物表面活性剂与环脂肽类结构一致。XJ-21菌株与外源ZW-027菌株复配后,复配体系对油藏环境条件具有广泛适应性,不仅在温度为35~55℃的范围内可以产生物酶使胍胶冻胶破胶,还可在50℃下降低样品所在区块的原油黏度,降黏率可达95.45%。在水驱后,使用复配体系驱油与仅用水驱技术相比可以提高10.14%的驱油率。During the process of reservoir fracturing modification,guanidine gum fracturing fluid is difficult to break gel at low temperature and has poor reservoir reconstruction effect,which needs to be solved urgently in reservoir development of Xinjiang Oilfield.Herein,microbial enhanced oil recovery technique was applied in guar gum fracturing fluid trying to solve these problems.The oil sludge was collected from the Shaofanggou group in Ji 7 block of Xinjiang Oilfield,and a strain named XJ-21,which was capable of producing high-yield surfactant,was obtained after enrichment culture,isolation and screening.This strain was identified as Bacillus subtilis based on its morphology,Gram Staining and 16S rRNA sequence analysis.Moreover,FTIR analysis revealed that the biosurfactant in XJ-21 metabolites had the same structure as cyclic lipopeptides.When XJ-21 strain was combined with an exogenous strain ZW-027,the formed compound system exhibited wide adaptability to reservoir environment,could produce biological enzymes to break guanidine gel in the temperature ranging 35~55℃,also could reduce the crude oil viscosity in the sample block at 50℃with the viscosity reduction rate reaching 95.45%.The oil displacement efficiency could be improved by 10.14%by flooding with the combination system compared with water flooding only.

关 键 词：压裂液 胍胶 微生物强化采油 稠油降黏 表面活性剂 油田化学品 

分 类 号：TE357.12[石油与天然气工程—油气田开发工程]