白芍标准煎液指纹图谱及化学成分指认  

Fingerprints of standardized decoction of radix Paeoniae Alba and identification of its chemical components

作　　者：于小红[1] 马致洁[1] 梅雪岭[2] Yu Xiaohong;Ma Zhijie;Mei Xueling(Department of Pharmacy,Beijing Friendship Hospital,Capital Medical University,Beijing,100050,China;Department of Education,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院药剂科,北京100050 [2]首都医科大学附属北京友谊医院教育处,北京100050

出　　处：《首都医科大学学报》2024年第2期289-295,共7页Journal of Capital Medical University

基　　金：国家重点研发计划资助项目(2023YFC2308200);北京市自然科学基金面上项目(7242041)。

摘　　要：目的建立白芍饮片标准煎液的指纹图谱,对白芍有效成分进行指认,以期为白芍饮片的质量控制及临床应用提供理论依据。方法收集10批白芍饮片,分别制备白芍标准煎液;采用超高效液相色谱(ultra performance liquid chromatography,UPLC)法建立白芍标准煎液的指纹图谱,以2012版《中药色谱指纹图谱相似度评价系统》进行指纹图谱相似度分析;通过与对照品比对、与文献核对质谱数据,对共有峰进行指认。结果经检测,10批白芍标准煎液指纹图谱共标定了22个共有峰,各峰分离度较好,各样品间相似度均>0.9;共指认出5个共有峰:儿茶素,芍药内酯苷,芍药苷,除虫菊素Ⅱ,苯甲酰芍药苷。结论该研究建立的指纹图谱方法精密度、重复性、稳定性良好,可为白芍饮片的质量评价提供理论参考。Objective To establish the fingerprints of the standard decoction of radix Paeoniae Alba,and to identify the active ingredients of radix Paeoniae Alba,in order to provide theoretical basis for the quality control and clinical application of radix Paeoniae Alba.Methods Ten batches of radix Paeoniae Alba were collected and the standard decoction of radix Paeoniae Alba was prepared;the fingerprints of the standard decoction of radix Paeoniae Alba were established by ultra performance liquid chromatography(UPLC),and the fingerprints were analyzed by the 2012 version of the Fingerprint Similarity Evaluation System for Traditional Chinese Medicine;and the common peaks were identified by comparing with the control product,and by checking the mass spectral data with the literature.Results The fingerprints of 10 batches of standard decoction of radix Paeoniae Alba were identified with 22 common peaks,and the peaks were well separated,with the similarity of each sample>0.9.Five common peaks were recognized,which were catechin,albiflora,paeoniflorin,pyrethrin Ⅱ,and benzoylpaeoniflorin,respectively.Conclusion The established fingerprint method has good precision,reproducibility and stability,and can provide a basis for the quality evaluation of radix Paeoniae Alba.

关 键 词：白芍 标准煎液 指纹图谱 相似度评价 成分指认 

分 类 号：R284[医药卫生—中药学]